Figure 6.
Metformin-based hydrogel scaffold to promote anti-tumor immune response of CAR-T cells. (A) Schematic illustration of metabolic reprogramming in CAR-T cells treated with the metformin-containing hydrogel. Created in https://BioRender.com. (B) Metabolic flux analysis of the oxygen consumption rate and the extracellular acidification rate in CAR-T cells treated with metformin, indicating suppression of glycolysis and enhancement in oxidative phosphorylation. (C) Upregulation of memory phenotype-related transcripts in CAR-T cells treated with CAR-T@Met/gel. (D) Bioluminescence images of CAR-T cell proliferation in primary and distant lesions in the HGC-27 tumor-bearing mice. Adapted with permission from 67, copyright 2023 Elsevier Ltd.