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. 1997 Dec 1;16(23):7019–7031. doi: 10.1093/emboj/16.23.7019

Control of B cell development by Ras-mediated activation of Raf.

B M Iritani 1, K A Forbush 1, M A Farrar 1, R M Perlmutter 1
PMCID: PMC1170305  PMID: 9384581

Abstract

Cell fate commitment in a variety of lineages requires signals conveyed via p21ras. To examine the role of p21ras in the development of B lymphocytes, we generated transgenic mice expressing a dominant-negative form of Ras in B lymphocyte progenitors, using a novel transcriptional element consisting of the Emu enhancer and the lck proximal promoter. Expression of dominant-negative Ras arrests B cell development at a very early stage, prior to formation of the pre-B cell receptor. Furthermore, an activated form of Raf expressed in the same experimental system could both drive the maturation of normal pro-B cells and rescue development of progenitors expressing dominant-negative Ras. Hence p21ras normally regulates early development of B lymphocytes by a mechanism that involves activation of the serine/threonine kinase Raf.

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Selected References

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