Abstract
Background
Neurodegenerative diseases, such as Alzheimer’s disease(AD), have become more prevalent in aging societies. Therefore, it is important to develop a blood‐based measurement system to diagnose early‐stage AD. However, such AD‐related biomarkers in blood exist at extremely low levels and are difficult to detect precisely and reproducibly.
Method
The measurement system was constructed by using brain natriuretic peptide(BNP) as a model biomarker. The outline of the system is as follows. (1) BNP in sample is captured by anti‐BNP antibody modified magnetic beads followed by enzyme labeling. (2) Magnetic beads with capturing BNP are enclosed in a microwell array for ultra‐sensitive detection of the marker. In our method, multiple beads are enclosed in a microwell to measure as many beads as possible and improve measurement reproducibility. (3) BNP is quantified by integrating the fluorescence intensity which generated by the enzymatic reaction in each microwells. BNP standards were measured in triplicate by developed system and calculated the limit of detection (LOD).
Result
The LOD of BNP was 2.5 fM (8.6 fg/mL), which was significantly sensitive compared to the conventional immunoassay system. Furthermore, the coefficient of variation (CV) of 7.2 fM (25 fg/mL) BNP was 8.1%, so that reproducibility in the low concentration range was also satisfactory.
Conclusion
We developed ultra‐sensitive and reproducible measurement system and it can be applied to the detection of AD‐related biomarkers in blood.