Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Oct;151(1):181–183. doi: 10.1042/bj1510181

An improved method for purifying sialidase.

M J Geisow
PMCID: PMC1172341  PMID: 174552

Abstract

An adsorbent specific for sialidase (EC 3.2.1.18) was prepared by coupling a glycoprotein containing glycosidically linked sialic acid to Sepharose. This adsorbent does not display the non-specific adsorption that occurs in previously reported methods.

Full text

PDF
181

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker R., Olsen K. W., Shaper J. H., Hill R. L. Agarose derivatives of uridine diphosphate and N-acetylglucosamine for the purification of a galactosyltransferase. J Biol Chem. 1972 Nov 25;247(22):7135–7147. [PubMed] [Google Scholar]
  3. Cuatrecasas P., Illiano G. Purification of neuraminidases from Vibrio Cholerae, Clostridium Perfringens and influenza virus by affinity chromatography. Biochem Biophys Res Commun. 1971 Jul 2;44(1):178–184. doi: 10.1016/s0006-291x(71)80175-4. [DOI] [PubMed] [Google Scholar]
  4. Huang C. C., Aminoff D. Non-specific protein binding by adsorbents designed for the specific affinity chromatography of sialidase in crude bacterial extracts. Biochim Biophys Acta. 1974 Dec 18;371(2):462–469. doi: 10.1016/0005-2795(74)90042-7. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
  7. Pastan I., Macchia V., Katzen R. A phospholipase specific for sphingomyelin from Clostridium perfringens. J Biol Chem. 1968 Jul 10;243(13):3750–3755. [PubMed] [Google Scholar]
  8. Spiro R. G. Glycoproteins. Annu Rev Biochem. 1970;39:599–638. doi: 10.1146/annurev.bi.39.070170.003123. [DOI] [PubMed] [Google Scholar]
  9. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES