Abstract
An adsorbent specific for sialidase (EC 3.2.1.18) was prepared by coupling a glycoprotein containing glycosidically linked sialic acid to Sepharose. This adsorbent does not display the non-specific adsorption that occurs in previously reported methods.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barker R., Olsen K. W., Shaper J. H., Hill R. L. Agarose derivatives of uridine diphosphate and N-acetylglucosamine for the purification of a galactosyltransferase. J Biol Chem. 1972 Nov 25;247(22):7135–7147. [PubMed] [Google Scholar]
- Cuatrecasas P., Illiano G. Purification of neuraminidases from Vibrio Cholerae, Clostridium Perfringens and influenza virus by affinity chromatography. Biochem Biophys Res Commun. 1971 Jul 2;44(1):178–184. doi: 10.1016/s0006-291x(71)80175-4. [DOI] [PubMed] [Google Scholar]
- Huang C. C., Aminoff D. Non-specific protein binding by adsorbents designed for the specific affinity chromatography of sialidase in crude bacterial extracts. Biochim Biophys Acta. 1974 Dec 18;371(2):462–469. doi: 10.1016/0005-2795(74)90042-7. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
- Pastan I., Macchia V., Katzen R. A phospholipase specific for sphingomyelin from Clostridium perfringens. J Biol Chem. 1968 Jul 10;243(13):3750–3755. [PubMed] [Google Scholar]
- Spiro R. G. Glycoproteins. Annu Rev Biochem. 1970;39:599–638. doi: 10.1146/annurev.bi.39.070170.003123. [DOI] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]