Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Nov;151(2):463–465. doi: 10.1042/bj1510463

A 30 S precursor of 30 S ribosomes in a mutant of Escherichia coli.

F Markey, D G Wild
PMCID: PMC1172382  PMID: 766751

Abstract

Escherichia coli 15-28, a mutant with a defect in ribosome metabolism, accumulates a ribonucleoprotein particle that is indistinguishable from 30S subunits by sedimentation but contains the precursor form of 16S RNA. This particle is probably a precursor of 30 S ribosomes.

Full text

PDF
463

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blundell M. R., Wild D. G. Inhibition of bacterial growth by metal salts. A survey of effects on the synthesis of ribonucleic acid and protein. Biochem J. 1969 Nov;115(2):207–212. doi: 10.1042/bj1150207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blundell M. R., Wild D. G. Inhibition of bacterial growth by metal salts. The accumulation of ribonucleic acid during inhibition of Escherichia coli by cobalt chloride. Biochem J. 1969 Nov;115(2):213–223. doi: 10.1042/bj1150213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hayes F., Hayes D. H. Biosynthesis of ribosomes in E. coli. I. Properties of ribosomal precursor particles and their RNA components. Biochimie. 1971;53(3):369–382. doi: 10.1016/s0300-9084(71)80104-9. [DOI] [PubMed] [Google Scholar]
  4. Hecht N. B., Woese C. R. Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis. J Bacteriol. 1968 Mar;95(3):986–990. doi: 10.1128/jb.95.3.986-990.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lindahl L. Intermediates and time kinetics of the in vivo assembly of Escherichia coli ribosomes. J Mol Biol. 1975 Feb 15;92(1):15–37. doi: 10.1016/0022-2836(75)90089-3. [DOI] [PubMed] [Google Scholar]
  6. Lindahl L. Two new ribosomal precursor particles in E. coli. Nat New Biol. 1973 Jun 6;243(127):170–172. doi: 10.1038/newbio243170a0. [DOI] [PubMed] [Google Scholar]
  7. MacDonald R. E., Turnock G., Forchhammer J. The synthesis and function of ribosomes in a new mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1967 Jan;57(1):141–147. doi: 10.1073/pnas.57.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mangiarotti G., Apirion D., Schlessinger D., Silengo L. Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli. Biochemistry. 1968 Jan;7(1):456–472. doi: 10.1021/bi00841a058. [DOI] [PubMed] [Google Scholar]
  9. Mangiarotti G., Turco E., Ponzetto A., Altruda F. Precursor 16S RNA in active 30S ribosomes. Nature. 1974 Jan 18;247(5437):147–148. doi: 10.1038/247147a0. [DOI] [PubMed] [Google Scholar]
  10. Margolis J., Kenrick K. G. Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Anal Biochem. 1968 Oct 24;25(1):347–362. doi: 10.1016/0003-2697(68)90109-7. [DOI] [PubMed] [Google Scholar]
  11. Nashimoto H., Nomura M. Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1440–1447. doi: 10.1073/pnas.67.3.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Osawa S., Otaka E., Itoh T., Fukui T. Biosynthesis of 50 s ribosomal subunit in Escherichia coli. J Mol Biol. 1969 Mar 28;40(3):321–351. doi: 10.1016/0022-2836(69)90158-2. [DOI] [PubMed] [Google Scholar]
  13. Peacock A. C., Dingman C. W. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. doi: 10.1021/bi00858a033. [DOI] [PubMed] [Google Scholar]
  14. Rosset R., Vola C., Feunteun J., Monier R. A Thermosensitive mutant defective in ribosomal 30 S subunit assembly. FEBS Lett. 1971 Oct 15;18(1):127–129. doi: 10.1016/0014-5793(71)80426-x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES