Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1975 Nov;152(2):389–392. doi: 10.1042/bj1520389

Inhibition of fatty acid synthesis and stimulation of glycogen breakdown by vasopressin in the perfused mouse liver

Gary Y Ma 1, Douglas A Hems 1
PMCID: PMC1172482  PMID: 1220692

Abstract

1. Vasopressin (anti-diuretic hormone, [8-arginine]vasopressin) inhibited the synthesis de novo of fatty acids (measured with 3H2O and U-14C-labelled lactate or U-14C-labelled glucose) and stimulated glycogen breakdown in the perfused liver of fed mice. 2. The concentration dependence of these effects (range 200–1000μunits/ml, i.e. 0.5–2.5ng/ml) resembled that for the action on glycogen breakdown which was previously reported for rat liver. 3. The appearance of newly synthesized fatty acids in both phospholipids and triglycerides was inhibited by vasopressin, whereas synthesis of cholesterol was less affected. 4. Inhibition of hepatic lipogenesis by vasopressin is the most potent short-term hormonal action on this process yet reported. Aspects of the effect are discussed, including the lack of a role for cyclic AMP, and a possible link with vasopressin action on glycogen metabolism.

Full text

PDF
389

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunengraber H., Boutry M., Lowenstein J. M. Fatty acid and 3- -hydroxysterol synthesis in the perfused rat liver. Including measurements on the production of lactate, pyruvate, -hydroxy-butyrate, and acetoacetate by the fed liver. J Biol Chem. 1973 Apr 25;248(8):2656–2669. [PubMed] [Google Scholar]
  2. Brunengraber H., Sabine J. R., Boutry M., Lowenstein J. M. 3- -Hydroxysterol synthesis by the liver. Arch Biochem Biophys. 1972 Jun;150(2):392–396. doi: 10.1016/0003-9861(72)90054-9. [DOI] [PubMed] [Google Scholar]
  3. Clark D. G., Rognstad R., Katz J. Lipogenesis in rat hepatocytes. J Biol Chem. 1974 Apr 10;249(7):2028–2036. [PubMed] [Google Scholar]
  4. Elliott J., Hems D. A., Beloff-Chain A. Carbohydrate metabolism of the isolated perfused liver of normal and genetically obese--hyperglycaemic (ob-ob) mice. Biochem J. 1971 Dec;125(3):773–780. doi: 10.1042/bj1250773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hems D. A., Rath E. A., Verrinder T. R. Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochem J. 1975 Aug;150(2):167–173. doi: 10.1042/bj1500167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hems D. A., Whitton P. D. Stimulation by vasopressin of glycogen breakdown and gluconeogenesis in the perfused rat liver. Biochem J. 1973 Nov;136(3):705–709. doi: 10.1042/bj1360705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keppens S., de Wulf H. The activation of liver glycogen phosphorylase by vasopressin. FEBS Lett. 1975 Mar 1;51(1):29–32. doi: 10.1016/0014-5793(75)80848-9. [DOI] [PubMed] [Google Scholar]
  8. Kirk C. J., Hems D. A. Hepatic action of vasopressin: lack of a role for adenosine-3',5'-cyclic monophosphate. FEBS Lett. 1974 Oct 1;47(1):128–131. doi: 10.1016/0014-5793(74)80441-2. [DOI] [PubMed] [Google Scholar]
  9. Raskin P., McGarry J. D., Foster D. W. Independence of cholesterol and fatty acid biosynthesis from cyclic adenosine monophosphate concentration in the perfused rat liver. J Biol Chem. 1974 Oct 10;249(19):6029–6032. [PubMed] [Google Scholar]
  10. Salmon D. M., Bowen N. L., Hems D. A. Synthesis of fatty acids in the perused mouse liver. Biochem J. 1974 Sep;142(3):611–618. doi: 10.1042/bj1420611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salmon D. M., Hems D. A. Plasma lipoproteins and the synthesis and turnover of plasma triglyceride in normal and genetically obese mice. Biochem J. 1973 Nov;136(3):551–563. doi: 10.1042/bj1360551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thurman R. G., Scholz R. Interaction of mixed-function oxidation with biosynthetic processes. 2. Inhibition of lipogenesis by aminopyrine in perfused rat liver. Eur J Biochem. 1973 Sep 21;38(1):73–78. doi: 10.1111/j.1432-1033.1973.tb03035.x. [DOI] [PubMed] [Google Scholar]
  13. Wadke M., Brunengraber H., Lowenstein J. M., Dolhun J. J., Arsenault G. P. Fatty acid synthesis by liver perfused with deuterated and tritiated water. Biochemistry. 1973 Jul 3;12(14):2619–2624. doi: 10.1021/bi00738a011. [DOI] [PubMed] [Google Scholar]
  14. Windmueller H. G., Spaeth A. E. De novo synthesis of fatty acid in perfused rat liver as a determinant of plasma lipoprotein production. Arch Biochem Biophys. 1967 Nov;122(2):362–369. doi: 10.1016/0003-9861(67)90206-8. [DOI] [PubMed] [Google Scholar]
  15. Windmueller H. G., Spaeth A. E. Perfusion in situ with tritium oxide to measure hepatic lipogenesis and lipid secretion. Normal and orotic acid-fed rats. J Biol Chem. 1966 Jun 25;241(12):2891–2899. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES