Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Dec;152(3):717–720. doi: 10.1042/bj1520717

Colchicine inhibition of the heparin-stimulated release of clearing-factor lipase from isolated fat-cells.

A Cryer, A McDonald, E R Williams, D S Robinson
PMCID: PMC1172531  PMID: 1227510

Abstract

When isolated fat-cells are incubated at 25 degrees C in serum-based media containing glucose, insulin and heparin, the rise that occurs in the clearing-factor lipase activity of the incubation medium is inhibited by colchicine. The rise in the fat-cell clearing-factor lipase activity that occurs during similar incubations in the absence of heparin is not affected by colchicine.

Full text

PDF
717

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butcher F. R., Goldman R. H. Effect of cytochalasin B and colchicine on the stimulation of -amylase release from rat parotid tissue slices. Biochem Biophys Res Commun. 1972 Jul 11;48(1):23–29. doi: 10.1016/0006-291x(72)90338-5. [DOI] [PubMed] [Google Scholar]
  2. Chajek T., Stein O., Stein Y. Colchicine-induced inhibition of plasma lipoprotein lipase release in the intact rat. Biochim Biophys Acta. 1975 Jan 24;380(1):127–131. doi: 10.1016/0005-2760(75)90051-x. [DOI] [PubMed] [Google Scholar]
  3. Chajek T., Stein O., Stein Y. Interference with the transport of heparin-releasable lipoprotein lipase in the perfused rat heart by colchicine and vinblastine. Biochim Biophys Acta. 1975 May 22;388(2):260–268. doi: 10.1016/0005-2760(75)90131-9. [DOI] [PubMed] [Google Scholar]
  4. Cheng K., Katosoyannis P. G. The inhibition of sugar transport and oxidation in fat cell ghosts by colchicine. Biochem Biophys Res Commun. 1975 Jan 2;64(3):1069–1075. doi: 10.1016/0006-291x(75)90156-4. [DOI] [PubMed] [Google Scholar]
  5. Cryer A., Davies P., Williams E. R., Robinson D. S. The clearing-factor lipase activity of isolated fat-cells. Biochem J. 1975 Feb;146(2):481–488. doi: 10.1042/bj1460481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies P., Cryer A., Robinson D. S. Hormonal control of adipose tissue clearing factor lipase activity. FEBS Lett. 1974 Sep 1;45(1):271–275. doi: 10.1016/0014-5793(74)80860-4. [DOI] [PubMed] [Google Scholar]
  7. Douglas W. W., Sorimachi M. Colchicine inhibits adrenal medullary secretion evoked by acetylcholine without affecting that evoked by potassium. Br J Pharmacol. 1972 May;45(1):129–132. [PMC free article] [PubMed] [Google Scholar]
  8. Ehrlich H. P., Ross R., Bornstein P. Effects of antimicrotubular agents on the secretion of collagen. A biochemical and morphological study. J Cell Biol. 1974 Aug;62(2):390–405. doi: 10.1083/jcb.62.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Garfinkel A. S., Schotz M. C. Sequential induction of two species of lipoprotein lipase. Biochim Biophys Acta. 1973 Apr 13;306(1):128–133. doi: 10.1016/0005-2760(73)90216-6. [DOI] [PubMed] [Google Scholar]
  10. Lacy P. E., Howell S. L., Young D. A., Fink C. J. New hypothesis of insulin secretion. Nature. 1968 Sep 14;219(5159):1177–1179. doi: 10.1038/2191177a0. [DOI] [PubMed] [Google Scholar]
  11. Le Marchand Y., Patzelt C., Assimacopoulos-Jeannet F., Loten E. G., Jeanrenaud B. Evidence for a role of the microtubular system in the secretion of newly synthesized albumin and other proteins by the liver. J Clin Invest. 1974 Jun;53(6):1512–1517. doi: 10.1172/JCI107701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Le Marchand Y., Singh A., Assimacopoulos-Jeannet F., Orci L., Rouiller C., Jeanrenaud B. A role for the microtubular system in the release of very low density lipoproteins by perfused mouse livers. J Biol Chem. 1973 Oct 10;248(19):6862–6870. [PubMed] [Google Scholar]
  13. Loten E. G., Jeanrenaud B. Effects of cytochalasin B, colchicine and vincristine on the metabolism of isolated fat-cells. Biochem J. 1974 May;140(2):185–192. doi: 10.1042/bj1400185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mizel S. B., Wilson L. Nucleoside transport in mammalian cells. Inhibition by colchicine. Biochemistry. 1972 Jul 4;11(14):2573–2578. doi: 10.1021/bi00764a003. [DOI] [PubMed] [Google Scholar]
  15. Orr T. S., Hall D. E., Allison A. C. Role of contractile microfilaments in the release of histamine from mast cells. Nature. 1972 Apr 14;236(5346):350–351. doi: 10.1038/236350a0. [DOI] [PubMed] [Google Scholar]
  16. Redman C. M., Banerjee D., Howell K., Palade G. E. Colchicine inhibition of plasma protein release from rat hepatocytes. J Cell Biol. 1975 Jul;66(1):42–59. doi: 10.1083/jcb.66.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson D. S., Wing D. R. Studies on tissue clearing factor lipase related to its role in the removal of lipoprotein triglyceride from the plasma. Biochem Soc Symp. 1971;(33):123–135. [PubMed] [Google Scholar]
  18. Rossignol B., Herman G., Keryer G. Inhibition by colchicine of carbamylcholine induced glycoprotein secretion by the submaxillary gland. A possible mechanism of cholinergic induced protein secretion. FEBS Lett. 1972 Mar 15;21(2):189–194. doi: 10.1016/0014-5793(72)80134-0. [DOI] [PubMed] [Google Scholar]
  19. Schimmel R. J. Inhibition of free fatty acid mobilization by colchicine. J Lipid Res. 1974 May;15(3):206–210. [PubMed] [Google Scholar]
  20. Schotz M. C., Garfinkel A. S. Effect of nutrition on species of lipoprotein lipase. Biochim Biophys Acta. 1972 Aug 11;270(4):472–478. doi: 10.1016/0005-2760(72)90112-9. [DOI] [PubMed] [Google Scholar]
  21. Soifer D., Braun T., Hechter O. Insulin and microtubules in rat adipocytes. Science. 1971 Apr 16;172(3980):269–271. doi: 10.1126/science.172.3980.269. [DOI] [PubMed] [Google Scholar]
  22. Stein O., Sanger L., Stein Y. Colchicine-induced inhibition of lipoprotein and protein secretion into the serum and lack of interference with secretion of biliary phospholipids and cholesterol by rat liver in vivo. J Cell Biol. 1974 Jul;62(1):90–103. doi: 10.1083/jcb.62.1.90. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stewart J. E., Schotz M. C. Release of lipoprotein lipase activity from isolated fat cells. II. Effect of heparin. J Biol Chem. 1974 Feb 10;249(3):904–907. [PubMed] [Google Scholar]
  24. Trifaró J. M., Collier B., Lastowecka A., Stern D. Inhibition by colchicine and by vinblastine of acetylcholine-induced catecholamine release from the adrenal gland: an anticholinergic action, not an effect upon microtubules. Mol Pharmacol. 1972 Mar;8(2):264–267. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES