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. 1976 Mar 15;154(3):731–734. doi: 10.1042/bj1540731

Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12.

S J Gutowski, H Rosenberg
PMCID: PMC1172776  PMID: 133673

Abstract

1. Anaerobic uptake of proline requires either the presence of a coupled Mg2+-stimulated adenosine triphosphatase or anaerobic electron transport. 2. Anaerobic uptake of glutamine does not require anaerobic electron transport even in the absence of a coupled Mg+2-stimulated adenosine triphosphatase. 3. These results support previous suggestions [Berger (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1514--1518; Berger & Heppel (1974) J. Biol. Chem. 249, 7747-7755; Kobayashi, Kin & Anraku (1974) J. Biochem. (Tokyo) 76, 251-261] that two distinct mechanisms of energy coupling to active transport exist in Escherichia coli in that energization of anaerobic proline uptake requires the 'high-energy membrane state', whereas the energization of anaerobic glutamine uptake does not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes E. M., Jr, Kaback H. R. Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1190–1198. doi: 10.1073/pnas.66.4.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
  4. Butlin J. D., Cox G. B., Gibson F. Oxidative phosphorylation in Escherichia coli K-12: the genetic and biochemical characterisations of a strain carrying a mutation in the uncB gene. Biochim Biophys Acta. 1973 Feb 22;292(2):366–375. doi: 10.1016/0005-2728(73)90043-1. [DOI] [PubMed] [Google Scholar]
  5. Cowell J. L. Energetics of glycylglycine transport in Escherichia coli. J Bacteriol. 1974 Oct;120(1):139–146. doi: 10.1128/jb.120.1.139-146.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Curtis S. J. Mechanism of energy coupling for transport of D-ribose in Escherichia coli. J Bacteriol. 1974 Oct;120(1):295–303. doi: 10.1128/jb.120.1.295-303.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harold F. M. Conservation and transformation of energy by bacterial membranes. Bacteriol Rev. 1972 Jun;36(2):172–230. doi: 10.1128/br.36.2.172-230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaback H. R., Reeves J. P., Short S. A., Lombardi F. J. Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys. 1974 Jan;160(1):215–222. doi: 10.1016/s0003-9861(74)80028-7. [DOI] [PubMed] [Google Scholar]
  9. Kadner R. J., Winkler H. H. Energy coupling for methionine transport in Escherichia coli. J Bacteriol. 1975 Sep;123(3):985–991. doi: 10.1128/jb.123.3.985-991.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein W. L., Boyer P. D. Energization of active transport by Escherichia coli. J Biol Chem. 1972 Nov 25;247(22):7257–7265. [PubMed] [Google Scholar]
  11. Kobayashi H., Kin E., Anraku Y. Transport of sugars and amino acids in bacteria. X. Sources of energy and energy coupling reactions of the active transport systems for isoleucine and proline in E. coli. J Biochem. 1974 Aug;76(2):251–261. doi: 10.1093/oxfordjournals.jbchem.a130567. [DOI] [PubMed] [Google Scholar]
  12. Konings W. N., Kaback H. R. Anaerobic transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3376–3381. doi: 10.1073/pnas.70.12.3376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  14. Or A., Kanner B. I., Gutnick D. L. Active transport in mutants of Escherichia coli with alterations in the membrane ATPase complex. FEBS Lett. 1973 Sep 15;35(2):217–219. doi: 10.1016/0014-5793(73)80288-1. [DOI] [PubMed] [Google Scholar]
  15. Rosenberg H., Cox G. B., Butlin J. D., Gutowski S. J. Metabolite transport in mutants of Escherichia coli K12 defective in electron transport and coupled phosphorylation. Biochem J. 1975 Feb;146(2):417–423. doi: 10.1042/bj1460417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schairer H. U., Gruber D. Mutants of Escherichia coli K12 defective in oxidative phosphorylation. Eur J Biochem. 1973 Aug 17;37(2):282–286. doi: 10.1111/j.1432-1033.1973.tb02986.x. [DOI] [PubMed] [Google Scholar]
  17. Schairer H. U., Haddock B. A. -Galactoside accumulation in a Mg 2+ -,Ca 2+ -activated ATPase deficient mutant of E.coli. Biochem Biophys Res Commun. 1972 Aug 7;48(3):544–551. doi: 10.1016/0006-291x(72)90382-8. [DOI] [PubMed] [Google Scholar]
  18. Simoni R. D., Postma P. W. The energetics of bacterial active transport. Annu Rev Biochem. 1975;44:523–554. doi: 10.1146/annurev.bi.44.070175.002515. [DOI] [PubMed] [Google Scholar]
  19. van Thienen G., Postma P. W. Coupling between energy conservation and active transport of serine in Escherichia coli. Biochim Biophys Acta. 1973 Oct 25;323(3):429–440. doi: 10.1016/0005-2736(73)90188-0. [DOI] [PubMed] [Google Scholar]

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