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. 1976 May 1;155(2):265–271. doi: 10.1042/bj1550265

Human lysosomal elastase. Catalytic and immunological properties.

P M Starkey, A J Barrett
PMCID: PMC1172831  PMID: 938478

Abstract

1. The elastase of human spleen was shown to exhibit endopeptidase activity against azo-casein and elastin. 2. Activity against several synthetic substrates was detected, and benzyloxycarbonyl-L-alanine 2-naphthyl ester was found to be a good substrate for routine use. 3. The enzyme showed a broad pH optimum in the range of 8.2-9.2 against azo-casein and the synthetic substrate. 4. The effect of inhibitors on the spleen elastase showed it to be a serine proteinase with a specificity similar to that of porcine pancreatic elastase. 5. Specific antisera were raised against the enzyme, and it was shown to be immunologically identical with the lysosomal elastase of human neutrophil leucocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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