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. 1972 Jun;128(1):99–106. doi: 10.1042/bj1280099

Microbial metabolism of C1 and C2 compounds. The involvement of glycollate in the metabolism of ethanol and of acetate by Pseudomonas AM1

Patricia M Dunstan 1, C Anthony 1, W T Drabble 1
PMCID: PMC1173574  PMID: 5085665

Abstract

Pseudomonas AM1 grows on ethanol with a mean generation time of about 10h. A single alcohol dehydrogenase is responsible for oxidation of both methanol and ethanol. It is proposed that the glyoxylate bypass does not operate in Pseudomonas AM1 during growth on ethanol. Although malate synthase is present in extracts of ethanol-grown Pseudomonas AM1, the activity of isocitrate lyase is negligible. Short-term incubation experiments with [14C]ethanol and [14C]acetate indicate that a novel pathway operates during growth of Pseudomonas AM1 on ethanol. Glycollate, glyoxylate and malate are probably intermediates in this pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony C., Zatman L. J. The microbial oxidation of methanol. 1. Isolation and properties of Pseudomonas sp. M27. Biochem J. 1964 Sep;92(3):609–614. doi: 10.1042/bj0920609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anthony C., Zatman L. J. The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27. Biochem J. 1965 Sep;96(3):808–812. doi: 10.1042/bj0960808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackmore M. A., Quayle J. R. Microbial growth on oxalate by a route not involving glyoxylate carboligase. Biochem J. 1970 Jun;118(1):53–59. doi: 10.1042/bj1180053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butlin J. D., Cox G. B., Gibson F. Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1971 Aug;124(1):75–81. doi: 10.1042/bj1240075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunstan P. M., Anthony C., Drabble W. T. Microbial metabolism of C 1 and C 2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1 compounds. Biochem J. 1972 Jun;128(1):107–115. doi: 10.1042/bj1280107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harder W., Quayle J. R. Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds. Biochem J. 1971 Mar;121(5):763–769. doi: 10.1042/bj1210763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heptinstall J., Quayle J. R. Pathways leading to and from serine during growth of Pseudomonas AM1 on C1 compounds or succinate. Biochem J. 1970 Apr;117(3):563–572. doi: 10.1042/bj1170563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson P. A., Quayle J. R. Microbial growth on C-1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM-1. Biochem J. 1964 Nov;93(2):281–290. doi: 10.1042/bj0930281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KORNBERG H. L., GOTTO A. M. The metabolism of C2 compounds in micro-organisms. 6. Synthesis of cell constituents from glycollate by Pseudomonas sp. Biochem J. 1961 Jan;78:69–82. doi: 10.1042/bj0780069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KORNBERG H. L., MORRIS J. G. Enzymic formation of oxaloacetate from erythro beta-hydroxyaspartate. Biochim Biophys Acta. 1962 Dec 17;65:537–540. doi: 10.1016/0006-3002(62)90467-5. [DOI] [PubMed] [Google Scholar]
  11. KORNBERG H. L. The metabolism of C2 compounds in micro-organisms. I. The incorporation of [2-14C] acetate by Pseudomonas fluorescens, and by a Corynebacterium, grown on ammonium acetate. Biochem J. 1958 Mar;68(3):535–542. doi: 10.1042/bj0680535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Large P. J., Quayle J. R. Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J. 1963 May;87(2):386–396. doi: 10.1042/bj0870386. [DOI] [PMC free article] [PubMed] [Google Scholar]

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