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. 1972 Jun;128(2):193–198. doi: 10.1042/bj1280193a

Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid

W D Sutton 1,*, P M B Walker 1
PMCID: PMC1173754  PMID: 5084787

Abstract

Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S1 followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K., PETERSEN G. B. The frequencies of certain sequences of nucleotides in deoxyribonucleic acid. Biochem J. 1960 Apr;75:17–27. doi: 10.1042/bj0750017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernardi G. Chromatography of nucleic acids on hydroxyapatite. Nature. 1965 May 22;206(4986):779–783. doi: 10.1038/206779a0. [DOI] [PubMed] [Google Scholar]
  3. Flamm W. G., McCallum M., Walker P. M. The isolation of complementary strands from a mouse DNA fraction. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1729–1734. doi: 10.1073/pnas.57.6.1729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Flamm W. G., Walker P. M., McCallum M. Renaturation and isolation of single strands from the nuclear DNA of the guinea pig. J Mol Biol. 1969 Jun 28;42(3):441–455. doi: 10.1016/0022-2836(69)90235-6. [DOI] [PubMed] [Google Scholar]
  5. Flamm W. G., Walker P. M., McCallum M. Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus). J Mol Biol. 1969 Mar 28;40(3):423–443. doi: 10.1016/0022-2836(69)90163-6. [DOI] [PubMed] [Google Scholar]
  6. MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
  7. McCallum M., Walker P. M. Hydroxyapatite fractionation procedures in the study of the mammalian genome. Biochem J. 1967 Oct;105(1):163–169. doi: 10.1042/bj1050163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pyeritz R. E., Lee C. S., Thomas C. A., Jr The cyclization of mouse satellite DNA. Chromosoma. 1971;33(3):284–296. doi: 10.1007/BF00284946. [DOI] [PubMed] [Google Scholar]
  9. Southern E. M. Base sequence and evolution of guinea-pig alpha-satellite DNA. Nature. 1970 Aug 22;227(5260):794–798. doi: 10.1038/227794a0. [DOI] [PubMed] [Google Scholar]
  10. Sutton W. D. A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochim Biophys Acta. 1971 Jul 29;240(4):522–531. doi: 10.1016/0005-2787(71)90709-x. [DOI] [PubMed] [Google Scholar]
  11. Sutton W. D., McCallum M. Mismatching and the reassociation rate of mouse satellite DNA. Nat New Biol. 1971 Jul 21;232(29):83–85. doi: 10.1038/newbio232083a0. [DOI] [PubMed] [Google Scholar]
  12. Waring M., Britten R. J. Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA. Science. 1966 Nov 11;154(3750):791–794. doi: 10.1126/science.154.3750.791. [DOI] [PubMed] [Google Scholar]
  13. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]

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