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. 1972 Jul;128(3):499–508. doi: 10.1042/bj1280499

The antibody binding site. Labelling of a specific antibody against the photo-precursor of an aryl nitrene

G W J Fleet *,†, J R Knowles †, R R Porter *
PMCID: PMC1173802  PMID: 4117794

Abstract

The isolation of specific rabbit antibodies for the haptenic group 4-azido-2-nitrophenyl, is described. These antibodies bind 1.8–2.0mol of hapten [∈-(4-azido-2-nitrophenyl)-l-lysine]/mol with an association constant of nearly 107m−1 at 4°C. On photolysis of the antibody–hapten complex, resulting in the formation of an aryl nitrene at the binding site, hapten was covalently bound to the antibody, and the antibody binding site was blocked. The ratio of labelling of heavy- and light-chains was 2.5:1. Two small peptides were isolated from digests of labelled heavy-chain, indicating that some 13% of the label in the antibody was attached to cysteine-92 and to alanine-93. These residues are adjacent to the major hypervariable region in rabbit heavy-chain (residues 95–105).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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