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. 1972 Jul;128(4):941–944. doi: 10.1042/bj1280941

The number of neurophysins in the rat. Influence of the concentration of Bromophenol Blue, used as a tracking dye, on the resolution of proteins by polyacrylamide-gel electrophoresis

G D Burford 1, B T Pickering 1
PMCID: PMC1173913  PMID: 4638797

Abstract

1. The concentration of Bromophenol Blue used as tracking dye in polyacrylamide-gel electrophoresis affected the resolution of rat neurophysins. 2. A final dye concentration of 1μg/ml in the tris–glycine running buffer was found to give the best results. 3. The presence of two major and one minor neurophysin(s) in the rat was confirmed. 4. The two major proteins were found to re-run as single discrete bands, which had the same mobilities in the absence of dye and different mobilities in its presence.

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Selected References

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  1. BARKA T. Studies of acid phosphatase. I. Electrophoretic separation of acid phosphatases of rat liver on polyacrylamide gels. J Histochem Cytochem. 1961 Sep;9:542–547. doi: 10.1177/9.5.542. [DOI] [PubMed] [Google Scholar]
  2. Burford G. D., Ginsburg M., Thomas P. J. The effect of denaturants and Ca2+ on the molecular weight and polymerisation of neurophysin. Biochim Biophys Acta. 1971 Mar 23;229(3):730–738. doi: 10.1016/0005-2795(71)90291-1. [DOI] [PubMed] [Google Scholar]
  3. Burford G. D., Jones C. W., Pickering B. T. Tentative identification of a vasopressin-neurophysin and an oxytocin-neurophysin in the rat. Biochem J. 1971 Oct;124(4):809–813. doi: 10.1042/bj1240809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CANN J. R., GOAD W. B. PRECAUTIONS REQUIRED IN INTERPRETATION OF MOVING-BOUNDARY AND ZONES ELECTROPHORETIC PATTERNS. Arch Biochem Biophys. 1964 Oct;108:171–172. doi: 10.1016/0003-9861(64)90369-8. [DOI] [PubMed] [Google Scholar]
  5. CLARKE J. T. SIMPLIFIED "DISC" (POLYACRYLAMIDE GEL) ELECTROPHORESIS. Ann N Y Acad Sci. 1964 Dec 28;121:428–436. doi: 10.1111/j.1749-6632.1964.tb14214.x. [DOI] [PubMed] [Google Scholar]
  6. Coy D. H., Wuu T. C. Purification and amino acid composition of constituents of rat neurophysin. Biochim Biophys Acta. 1972 Mar 15;263(1):125–132. doi: 10.1016/0005-2795(72)90166-3. [DOI] [PubMed] [Google Scholar]
  7. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  8. Hartman B. K., Udenfriend S. A method for immediate visualization of proteins in acrylamide gels and its use for preparation of antibodies to enzymes. Anal Biochem. 1969 Sep;30(3):391–394. doi: 10.1016/0003-2697(69)90132-8. [DOI] [PubMed] [Google Scholar]
  9. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Norström A., Sjöstrand J., Livett B. G., Uttenthal L. O., Hope D. B. Electrophoretic and immunological characterization of rat neurophysin. Biochem J. 1971 May;122(5):671–676. doi: 10.1042/bj1220671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rauch R., Hollenberg M. D., Hope D. B. Isolation of a third bovine neurophysin. Biochem J. 1969 Nov;115(3):473–479. doi: 10.1042/bj1150473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sachs H., Fawcett P., Takabatake Y., Portanova R. Biosynthesis and release of vasopressin and neurophysin. Recent Prog Horm Res. 1969;25:447–491. doi: 10.1016/b978-0-12-571125-8.50013-2. [DOI] [PubMed] [Google Scholar]
  13. Uttenthal L. O., Hope D. B. The isolation of three neurophysins from porcine posterior pituitary lobes. Biochem J. 1970 Mar;116(5):899–909. doi: 10.1042/bj1160899. [DOI] [PMC free article] [PubMed] [Google Scholar]

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