Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1972 Sep;129(2):231–239. doi: 10.1042/bj1290231

A study of stabilization of gluconeogenic activity in rat liver slices by calcium and manganese ions

Anne Roobol 1, G A O Alleyne 1
PMCID: PMC1174073  PMID: 4643308

Abstract

1. The effect of some bivalent cations on gluconeogenesis by the rat liver-slice preparation has been investigated. 2. Ca2+ and Mn2+ stimulated glucose production from a range of substrates but not from glycerol. Mg2+ had no effect on the rate of glucose production. 3. Ca2+ were required to maintain phosphoenolpyruvate carboxylase activity in the slice preparation. 4. Ca2+ and Mn2+, but not Mg2+, retarded the release of lysosomal enzymes from the slice into the incubation medium. 5. It is proposed that Ca2+ and Mn2+ stimulate glucose production by stabilizing the lysosome system in the liver-slice preparation. 6. The value of the liver-slice preparation as a means of measuring hepatic gluconeogenesis is discussed.

Full text

PDF
231

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashmore J., Wagle S. R., Uete T. Studies on gluconeogenesis. Adv Enzyme Regul. 1964;2:101–114. doi: 10.1016/s0065-2571(64)80008-x. [DOI] [PubMed] [Google Scholar]
  2. Exton J. H., Park C. R. Control of gluconeogenesis in liver. I. General features of gluconeogenesis in the perfused livers of rats. J Biol Chem. 1967 Jun 10;242(11):2622–2636. [PubMed] [Google Scholar]
  3. Flores H., Alleyne G. A. Phosphoenolpyruvate carboxykinase of kidney. Subcellular distribution and response to acid-base changes. Biochem J. 1971 Jun;123(1):35–39. doi: 10.1042/bj1230035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedmann N., Rasmussen H. Calcium, manganese and hepatic gluconeogenesis. Biochim Biophys Acta. 1970 Oct 27;222(1):41–52. doi: 10.1016/0304-4165(70)90349-1. [DOI] [PubMed] [Google Scholar]
  5. GIANETTO R., DE DUVE C. Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles. Biochem J. 1955 Mar;59(3):433–438. doi: 10.1042/bj0590433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gevers W., Krebs H. A. The effects of adenine nucleotides on carbohydrate metabolism in pigeon-liver homogenates. Biochem J. 1966 Mar;98(3):720–735. doi: 10.1042/bj0980720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HAYNES R. C., Jr Studies of an vitro effect of glucocorticoids on gluconeogenesis. Endocrinology. 1962 Sep;71:399–406. doi: 10.1210/endo-71-3-399. [DOI] [PubMed] [Google Scholar]
  8. HUGGETT A. S., NIXON D. A. Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose. Lancet. 1957 Aug 24;273(6991):368–370. doi: 10.1016/s0140-6736(57)92595-3. [DOI] [PubMed] [Google Scholar]
  9. Hems R., Ross B. D., Berry M. N., Krebs H. A. Gluconeogenesis in the perfused rat liver. Biochem J. 1966 Nov;101(2):284–292. doi: 10.1042/bj1010284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson J. A., Fusaro R. M. The quantitative enzymic determination of animal liver glycogen. Anal Biochem. 1966 Apr;15(1):140–149. doi: 10.1016/0003-2697(66)90256-9. [DOI] [PubMed] [Google Scholar]
  11. KREBS H. A., BENNETT D. A., DE GASQUET P., GASQUET P., GASCOYNE T., YOSHIDA T. Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices. Biochem J. 1963 Jan;86:22–27. doi: 10.1042/bj0860022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kamm D. E., Cahill G. F., Jr Effect of acid-base status on renal and hepatic gluconeogenesis in diabetes and fasting. Am J Physiol. 1969 May;216(5):1207–1212. doi: 10.1152/ajplegacy.1969.216.5.1207. [DOI] [PubMed] [Google Scholar]
  13. Kimmich G. A., Rasmussen H. Regulation of pyruvate carboxylase activity by calcium in intact rat liver mitochondria. J Biol Chem. 1969 Jan 10;244(1):190–199. [PubMed] [Google Scholar]
  14. Krebs H. A., Notton B. M., Hems R. Gluconeogenesis in mouse-liver slices. Biochem J. 1966 Dec;101(3):607–617. doi: 10.1042/bj1010607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Krebs H. A. Rate control of the tricarboxylic acid cycle. Adv Enzyme Regul. 1970;8:335–353. doi: 10.1016/0065-2571(70)90028-2. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lueck J. D., Miller L. L. The effect of perfusate pH on glutamine metabolism in the isolated perfused rat liver. J Biol Chem. 1970 Oct 25;245(20):5491–5497. [PubMed] [Google Scholar]
  18. MUSA B. U., DOE R. P., SEAL U. S. PURIFICATION AND PROPERTIES OF HUMAN LIVER BETA-GLUCURONIDASE. J Biol Chem. 1965 Jul;240:2811–2816. [PubMed] [Google Scholar]
  19. Papavasiliou P. S., Miller S. T., Cotzias G. C. Functional interactions between biogenic amines, 3',5'-cyclic AMP and manganese. Nature. 1968 Oct 5;220(5162):74–75. doi: 10.1038/220074a0. [DOI] [PubMed] [Google Scholar]
  20. Ross B. D., Hems R., Krebs H. A. The rate of gluconeogenesis from various precursors in the perfused rat liver. Biochem J. 1967 Mar;102(3):942–951. doi: 10.1042/bj1020942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SIMPSON M. V. The release of labeled amino acids from the proteins of rat liver slices. J Biol Chem. 1953 Mar;201(1):143–154. [PubMed] [Google Scholar]
  22. STEINBERG D., VAUGHAN M. Intracellular protein degradation in vitro. Biochim Biophys Acta. 1956 Mar;19(3):584–585. doi: 10.1016/0006-3002(56)90504-2. [DOI] [PubMed] [Google Scholar]
  23. Schimke R. T., Sweeney E. W., Berlin C. M. Studies of the stability in vivo and in vitro of rat liver tryptophan pyrrolase. J Biol Chem. 1965 Dec;240(12):4609–4620. [PubMed] [Google Scholar]
  24. Schimmel R. J., Knobil E. Role of free fatty acids in stimulation of gluconeogenesis during fasting. Am J Physiol. 1969 Dec;217(6):1803–1808. doi: 10.1152/ajplegacy.1969.217.6.1803. [DOI] [PubMed] [Google Scholar]
  25. WEISSMANN G. LABILIZATION AND STABILIZATION OF LYSOSOMES. Fed Proc. 1964 Sep-Oct;23:1038–1044. [PubMed] [Google Scholar]
  26. WEISSMANN G., THOMAS L. THE EFFECTS OF CORTICOSTEROIDS UPON CONNECTIVE TISSUE AND LYSOSOMES. Recent Prog Horm Res. 1964;20:215–245. [PubMed] [Google Scholar]
  27. Wagle S. R., Gaskins R. K., Jacoby A., Ashmore J. Studies on glucose synthesis by rat liver and kidney cortex slices. Life Sci. 1966 Apr;5(7):655–663. doi: 10.1016/0024-3205(66)90297-9. [DOI] [PubMed] [Google Scholar]
  28. Wimhurst Janet M., Manchester K. L. Role of bivalent cations in the control of enzymes involved in gluconeogenesis. FEBS Lett. 1970 Sep 18;10(1):33–37. doi: 10.1016/0014-5793(70)80409-4. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES