Abstract
1. Glucose oxidase (EC 1.1.3.4) and urease (EC 3.5.1.5) were covalently attached through glutaraldehyde to low-molecular-weight nylon powder. 2. Immobilized derivatives of glucose oxidase and urease were prepared by cross-linking the respective enzymes within the matrix of a nylon membrane. 3. An improved process is described for the immobilization of glucose oxidase and urease on the inside surface of partially hydrolysed nylon tube. 4. Automated analytical procedures are described for the determination of glucose with each of the three immobilized glucose oxidase derivatives and for the determination of urea with each of the three immobilized urease derivatives. 5. The efficiencies of the three immobilized enzyme structures as reagents for the automated determination of their substrates were compared.
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Selected References
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- BEERS R. F., Jr, SIZER I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed] [Google Scholar]
- CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
- Filippusson H., Hornby W. E., McDonald A. The use of immobilised derivatives of urease and urate oxidase in automated analysis. FEBS Lett. 1972 Feb 15;20(3):291–293. doi: 10.1016/0014-5793(72)80089-9. [DOI] [PubMed] [Google Scholar]
- Hornby W. E., Filippusson H., McDonald A. The preparation of glucose oxidase chemically attached to polystyrene and its use in the automated analyses of glucose. FEBS Lett. 1970 Jul 15;9(1):8–10. doi: 10.1016/0014-5793(70)80297-6. [DOI] [PubMed] [Google Scholar]
- Jansen E. F., Tomimatsu Y., Olson A. C. Cross-linking of -chymotrypsin and other proteins by reaction with glutaraldehyde. Arch Biochem Biophys. 1971 May;144(1):394–400. doi: 10.1016/0003-9861(71)90492-9. [DOI] [PubMed] [Google Scholar]
- Sundaram P. V., Hornby W. E. Preparation and properties of urease chemically attached to nylon tube. FEBS Lett. 1970 Oct;10(5):325–327. doi: 10.1016/0014-5793(70)80464-1. [DOI] [PubMed] [Google Scholar]