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. 1972 Sep;129(2):355–365. doi: 10.1042/bj1290355

Modification by calcium ions of adenine nucleotide translocation in rat liver mitochondria

T Spencer 1, F L Bygrave 1
PMCID: PMC1174085  PMID: 4643322

Abstract

1. Added Ca2+ stimulates the translocation of ATP by isolated rat liver mitochondria. 2. The apparent Km for added Ca2+ in stimulating the translocation of 200μm-ATP is approx. 160μm (75μm `free' Ca2+). 3. The greatest stimulation of ATP translocation by Ca2+ occurs at the lower concentrations of ATP. 4. Sr2+ (and to a lesser extent Ba2+) can replace Ca2+ whereas Mg2+ and Mn2+ have only little ability to stimulate ATP translocation. 5. Translocation of dATP is also stimulated by Ca2+ whereas that of ADP is stimulated to only a relatively small degree. 6. Studies with metabolic inhibitors and uncouplers provide evidence that stimulation by Ca2+ and by uncouplers is additive and that the mechanism of Ca2+ stimulation does not seem to involve the high-energy intermediate of oxidative phosphorylation. 7. In the presence of Ca2+, ATP is able to effectively compete with ADP for translocation. 8. Added K+ further enhances the ability of Ca2+ to stimulate ATP translocation. 9. These findings are discussed in relation to the potential involvement of Ca2+ in modifying enzymic reactions involved in the regulation of cell metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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