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. 1972 Sep;129(3):645–655. doi: 10.1042/bj1290645

Interaction of soluble glucosyl- and mannosyl-transferase enzyme activities in the synthesis of a glucomannan

J S Heller 1, C L Villemez 1
PMCID: PMC1174166  PMID: 4658992

Abstract

A neutral-detergent-solubilized-enzyme preparation derived from Phaseolus aureus hypocotyls contains two types of glycosyltransferase activity. One, mannosyltransferase enzyme activity, utilizes GDP-α-d-mannose as the sugar nucleotide substrate. The other, glucosyltransferase enzyme activity, utilizes GDP-α-d-glucose as the sugar nucleotide substrate. The soluble enzyme preparation catalyses the formation of what appears to be a homopolysaccharide when either sugar nucleotide is the only substrate present. A β-(1→4)-linked mannan is the only polymeric product when only GDP-α-d-mannose is added. A β-(1→4)-linked glucan is the only polymeric product when only GDP-α-d-glucose is added. In the presence of both sugar nucleotides, however, a β-(1→4)-linked glucomannan is formed. There are indications that endogenous sugar donors may be present in the enzyme preparation. There appear to be only two glycosyltransferases in the enzyme preparation, each catalysing the transfer of a different sugar to the same type of acceptor molecule. The glucosyltransferase requires the continual production of mannose-containing acceptor molecules for maintenance of enzyme activity, and is thereby dependent upon the activity of the mannosyltransferase. The mannosyltransferase, on the other hand, does not require the continual production of glucose-containing acceptors for maintenance of enzyme activity, but is severely inhibited by GDP-α-P-glucose. These properties promote the synthesis of β-(1→4)-linked glucomannan rather than β-(1→4)-linked glucan plus β-(1→4)-linked mannan when both sugar nucleotide substrates are present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBER H. A., ELBEIN A. D., HASSID W. Z. THE SYNTHESIS OF CELLULOSE BY ENZYME SYSTEMS FROM HIGHER PLANTS. J Biol Chem. 1964 Dec;239:4056–4061. [PubMed] [Google Scholar]
  2. FEINGOLD D. S., NEUFELD E. F., HASSID W. Z. Synthesis of a beta-1, 3-linked glucan by extracts of Phaseolus aureus seedlings. J Biol Chem. 1958 Oct;233(4):783–788. [PubMed] [Google Scholar]
  3. Heller J. S., Villemez C. L. Solubilization of a mannose-polymerizing enzyme from Phaseolus aureus. Biochem J. 1972 Jun;128(2):243–252. doi: 10.1042/bj1280243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kauss H. A plant mannosyl-lipid acting in reversible transfer of mannose. FEBS Lett. 1969 Sep;5(1):81–84. doi: 10.1016/0014-5793(69)80298-x. [DOI] [PubMed] [Google Scholar]
  5. Liu T. Y., Hassid W. Z. Solubilization and partial purification of cellulose synthetase from Phaseolus aureus. J Biol Chem. 1970 Apr 25;245(8):1922–1925. [PubMed] [Google Scholar]
  6. REESE E. T., SHIBATA Y. BETA-MANNANASES OF FUNGI. Can J Microbiol. 1965 Apr;11:167–183. doi: 10.1139/m65-023. [DOI] [PubMed] [Google Scholar]
  7. Scher M., Lennarz W. J. Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. I. Characterization of mannan-14C formed enzymatically from mannosyl-1-phosphoryl-undecaprenol. J Biol Chem. 1969 May 25;244(10):2777–2789. [PubMed] [Google Scholar]
  8. Scher M., Lennarz W. J. Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. I. Characterization of mannan-14C formed enzymatically from mannosyl-1-phosphoryl-undecaprenol. J Biol Chem. 1969 May 25;244(10):2777–2789. [PubMed] [Google Scholar]
  9. Stewart T. S., Mendershausen P. B., Ballou C. E. Preparation of a mannopentaose, mannohexaose, and mannoheptaose from Saccharomyces cerevisiae mannan. Biochemistry. 1968 May;7(5):1843–1854. doi: 10.1021/bi00845a032. [DOI] [PubMed] [Google Scholar]
  10. Tsai C. M., Hassid W. Z. Solubilization and Separation of Uridine Diphospho-d-glucose: beta-(1 --> 4) Glucan and Uridine Diphospho-d-glucose:beta-(1 --> 3) Glucan Glucosyltransferases from Coleoptiles of Avena sativa. Plant Physiol. 1971 Jun;47(6):740–744. doi: 10.1104/pp.47.6.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Villemez C. L. Characterization of intermediates in plant cell wall biosynthesis. Biochem Biophys Res Commun. 1970 Aug 11;40(3):636–641. doi: 10.1016/0006-291x(70)90951-4. [DOI] [PubMed] [Google Scholar]
  12. Villemez C. L., Clark A. F. A particle bound intermediate in the biosynthesis of plant cell wall polysaccharides. Biochem Biophys Res Commun. 1969 Jul 7;36(1):57–63. doi: 10.1016/0006-291x(69)90649-4. [DOI] [PubMed] [Google Scholar]
  13. Villemez C. L. Rate studies of polysaccharide biosynthesis from guanosine diphosphate -D-glucose and guanosine diphosphate -D-mannose. Biochem J. 1971 Jan;121(1):151–157. doi: 10.1042/bj1210151. [DOI] [PMC free article] [PubMed] [Google Scholar]

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