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. 1972 Nov;130(1):281–288. doi: 10.1042/bj1300281

Amphibian ribosomal ribonucleic acids

M Elizabeth Rogers 1, Gerda Klein 1
PMCID: PMC1174325  PMID: 4655433

Abstract

1. The larger rRNA molecules of different species of amphibia do not all have the same mobility on polyacrylamide gels, and the difference appears to be one between the Anuran and Urodele amphibia. 2. Alteration of conditions of electrophoresis so as to distinguish between differences of conformation and molecular weight does not affect the results. 3. The mobility on gel electrophoresis of the rRNA precursor in two species of amphibia is similar, although the intermediate processing products differ. 4. It is suggested that the mobility differences observed represent differences of molecular weight, and are an example of the variability of the products of transcription and processing of rRNA genes in two amphibian genera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN D. D., GURDON J. B. ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS. Proc Natl Acad Sci U S A. 1964 Jan;51:139–146. doi: 10.1073/pnas.51.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnstiel M., Speirs J., Purdom I., Jones K., Loening U. E. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature. 1968 Aug 3;219(5153):454–463. doi: 10.1038/219454a0. [DOI] [PubMed] [Google Scholar]
  3. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  4. Dawid I. B., Brown D. D., Reeder R. H. Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis. J Mol Biol. 1970 Jul 28;51(2):341–360. doi: 10.1016/0022-2836(70)90147-6. [DOI] [PubMed] [Google Scholar]
  5. ELSDALE T. R., FISCHBERG M., SMITH S. A mutation that reduces nucleolar number in Xenopus laevis. Exp Cell Res. 1958 Jun;14(3):642–643. doi: 10.1016/0014-4827(58)90175-7. [DOI] [PubMed] [Google Scholar]
  6. Egawa K., Choi Y. C., Busch H. Studies on the role of 23 s nucleolar RNA as an intermediate in the synthesis of 18 s ribosomal RNA. J Mol Biol. 1971 Mar 28;56(3):565–577. doi: 10.1016/0022-2836(71)90402-5. [DOI] [PubMed] [Google Scholar]
  7. Eliceiri G. L., Green H. Ribosomal RNA synthesis in human-mouse hybrid cells. J Mol Biol. 1969 Apr;41(2):253–260. doi: 10.1016/0022-2836(69)90390-8. [DOI] [PubMed] [Google Scholar]
  8. Gall J. G. Nuclear RNA of the salamander oocyte. Natl Cancer Inst Monogr. 1966 Dec;23:475–488. [PubMed] [Google Scholar]
  9. Grierson D., Loening U. E. Distinct transcription products of ribosomal genes in two different tissues. Nat New Biol. 1972 Jan 19;235(55):80–82. doi: 10.1038/newbio235080a0. [DOI] [PubMed] [Google Scholar]
  10. Groot P. H., Aaij C., Borst P. Variation with temperature of the apparent molecular weight of rat-liver mitochondrial RNA, determined by gel electrophoresis. Biochem Biophys Res Commun. 1970 Dec 9;41(5):1321–1327. doi: 10.1016/0006-291x(70)90233-0. [DOI] [PubMed] [Google Scholar]
  11. Landesman R., Gross P. R. Patterns of macromolecule synthesis during development of Xenopus laevis. II. Identification of the 40 S precursor to ribosomal RNA. Dev Biol. 1969 Mar;19(3):244–260. doi: 10.1016/0012-1606(69)90063-3. [DOI] [PubMed] [Google Scholar]
  12. Loening U. E., Jones K. W., Birnstiel M. L. Properties of the ribosomal RNA precursor in Xenopus laevis; comparison to the precursor in mammals and in plants. J Mol Biol. 1969 Oct 28;45(2):353–366. doi: 10.1016/0022-2836(69)90110-7. [DOI] [PubMed] [Google Scholar]
  13. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  14. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Montenecourt B. S., Langsam M. E., Dubin D. T. Mitochondrial RNA from cultured animal cells. II. A comparison of the high molecular weight RNA from mouse and hamster cells. J Cell Biol. 1970 Aug;46(2):245–251. doi: 10.1083/jcb.46.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parish J. H., Kirby K. S. Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim Biophys Acta. 1966 Dec 21;129(3):554–562. doi: 10.1016/0005-2787(66)90070-0. [DOI] [PubMed] [Google Scholar]
  18. Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
  19. Perry R. P., Cheng T. Y., Freed J. J., Greenberg J. R., Kelley D. E., Tartof K. D. Evolution of the transcription unit of ribosomal RNA. Proc Natl Acad Sci U S A. 1970 Mar;65(3):609–616. doi: 10.1073/pnas.65.3.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reeder R. H., Brown D. D. Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium. J Mol Biol. 1970 Jul 28;51(2):361–377. doi: 10.1016/0022-2836(70)90148-8. [DOI] [PubMed] [Google Scholar]
  21. Rogers M. E., Loening U. E., Fraser R. S. Ribosomal RNA precursors in plants. J Mol Biol. 1970 May 14;49(3):681–692. doi: 10.1016/0022-2836(70)90291-3. [DOI] [PubMed] [Google Scholar]
  22. Rogers M. E. Ribonucleoprotein particles in the amphibian oocyte nucleus. Possible intermediates in ribosome synthesis. J Cell Biol. 1968 Mar;36(3):421–432. doi: 10.1083/jcb.36.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stanley W. M., Jr, Bock R. M. Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry. 1965 Jul;4(7):1302–1311. doi: 10.1021/bi00883a014. [DOI] [PubMed] [Google Scholar]
  24. Wallace H., Birnstiel M. L. Ribosomal cistrons and the nucleolar organizer. Biochim Biophys Acta. 1966 Feb 21;114(2):296–310. doi: 10.1016/0005-2787(66)90311-x. [DOI] [PubMed] [Google Scholar]
  25. Weinberg R. A., Loening U., Willems M., Penman S. Acrylamide gel electrophoresis of HeLa cell nucleolar RNA. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088–1095. doi: 10.1073/pnas.58.3.1088. [DOI] [PMC free article] [PubMed] [Google Scholar]

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