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. 1971 Jan;121(2):279–285. doi: 10.1042/bj1210279

Differences in the ribosomes prepared from lactating and non-lactating bovine mammary gland

M D Herrington 1, A O Hawtrey 1
PMCID: PMC1176567  PMID: 5165653

Abstract

1. Ribosomes prepared from bovine lactating mammary gland are able to synthesize protein, whereas similar preparations from non-lactating glands are not. Washing the ribosome suspensions through a medium containing 0.5m-ammonium chloride enhanced their ability to incorporate phenylalanine into polyphenylalanine. 2. Ribosomes isolated from non-lactating bovine mammary gland, in contrast with those from rat liver and lactating mammary gland, contained significant amounts of extraneous nucleases. These enzymes could be removed by washing with a medium A buffer containing 0.5m-ammonium chloride. 3. Only those ribosomes from functionally active tissues were able to bind polyuridylic acid and phenylalanyl-tRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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