Abstract
The amino acid sequences of sesame (Sesamum indicum L.) and castor (Ricinus communis L.) cytochrome c were determined by using 1.5μmol of protein from each species. Both molecules consist of a single chain of 111 amino acid residues and are homologous with other mitochondrial cytochrome c molecules. Both have an N-acetylated `tail' of eight amino acids and two ∈-N-trimethyl-lysine residues, as also reported for wheat germ (Delange, Glazer & Smith, 1969) and mung-bean cytochrome c (Thompson, Laycock, Ramshaw & Boulter, 1970). Two different preparations of castor cytochrome c differed by one residue. This was glutamic acid for glutamine in position 100. The results for sesame and castor cytochrome c led to a re-examination and subsequent correction to the N-terminal region of the mung-bean cytochrome c sequence, as given by Thompson et al. (1970).
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boulter D., Thompson E. W., Ramshaw J. A., Richardson M. Higher plant cytochrome c. Nature. 1970 Nov 7;228(5271):552–554. doi: 10.1038/228552a0. [DOI] [PubMed] [Google Scholar]
- DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
- Nolan C., Margoliash E. Primary structure of the cytochrome c from the great grey kangaroo, Macropus canguru. J Biol Chem. 1966 Mar 10;241(5):1049–1059. [PubMed] [Google Scholar]
- Ramshaw J. A., Thompson E. W., Boulter D. The amino acid sequence of Helianthus annuus L. (sunflower) cyrochrome c deduced from chymotryptic peptides. Biochem J. 1970 Sep;119(3):535–539. doi: 10.1042/bj1190535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmer G., Kreil G. Micro method for detection of formyl and acetyl groups in proteins. Anal Biochem. 1969 May;29(2):186–192. doi: 10.1016/0003-2697(69)90301-7. [DOI] [PubMed] [Google Scholar]
- Stevens F. C., Glazer A. N., Smith E. L. The amino acid sequence of wheat germ cytochrome c. J Biol Chem. 1967 Jun 10;242(11):2764–2779. [PubMed] [Google Scholar]
- Thompson E. W., Laycock M. V., Ramshaw J. A., Boulter D. The amino acid sequence of Phaseolus aureua L. (mung-bean) cytochrome c. Biochem J. 1970 Mar;117(1):183–192. doi: 10.1042/bj1170183. [DOI] [PMC free article] [PubMed] [Google Scholar]
