Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1971 Feb;121(3):461–467. doi: 10.1042/bj1210461

Properties of yeast grown anaerobically in media limiting in potassium

W Bartley 1, Valerie Broomhead 1
PMCID: PMC1176594  PMID: 4330378

Abstract

1. Saccharomyces cerevisiae was grown anaerobically in media with different concentrations of K+ down to less than 1mm. Below 3.2mm the K+ concentration limited the growth rate and yield. 2. Yeast extract was essential for maximum growth. The yield of cells when the medium contained 0.83mm-K+ was only 30% of the yield with 90mm-K+. 3. At the end of anaerobic growth the cells grown in 0.83mm-K+ had a higher concentration of oxidative enzymes than cells grown in 90mm-K+. 4. The cells grown anaerobically in 0.83mm-K+ could adapt to aerobic conditions if K+ was present in the adaptation medium, but not otherwise. 5. The enzyme pattern of the yeast grown aerobically in 0.83mm-K+ was very similar to the anaerobically grown cells and did not change markedly after the glucose was consumed.

Full text

PDF
461

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartley W., Tustanoff E. R. The effect of metabolic inhibitors on the development of respiration in anaerobically grown yeast. Biochem J. 1966 Jun;99(3):599–603. doi: 10.1042/bj0990599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAIX P., HEYMAN-BLANCHET T. Spectres cytochromiques de la levure cultivée en anaérobiose ou en aérobiose. Biochim Biophys Acta. 1957 Oct;26(1):214–215. doi: 10.1016/0006-3002(57)90080-x. [DOI] [PubMed] [Google Scholar]
  3. CONWAY E. J., DOWNEY M. An outer metabolic region of the yeast cell. Biochem J. 1950 Sep;47(3):347–355. doi: 10.1042/bj0470347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONWAY E. J., MOORE P. T. A sodium-yeast and some of its properties. Biochem J. 1954 Jul;57(3):523–528. doi: 10.1042/bj0570523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chapman C., Bartley W. The kinetics of enzyme changes in yeast under conditions that cause the loss of mitochondria. Biochem J. 1968 Apr;107(4):455–465. doi: 10.1042/bj1070455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HEBB C. R., SLEBODNIK J. The effect of prior growth conditions on the kinetics of adaptive enzyme formation in yeast. Exp Cell Res. 1958 Apr;14(2):286–294. doi: 10.1016/0014-4827(58)90186-1. [DOI] [PubMed] [Google Scholar]
  7. HEYMAN-BLANCHET T., CHAIX P. [Variations in the cytochromic spectrum of anaerobically cultured yeast as a function of its growth phases]. Biochim Biophys Acta. 1959 Sep;35:85–93. doi: 10.1016/0006-3002(59)90337-3. [DOI] [PubMed] [Google Scholar]
  8. HOLZER H., GOEDDE H. W. Oxydation von alpha-Ketosäuren und einigen Aldehyden mit Pyruvat-decarboxylase aus Hefe. Biochem Z. 1957;329(3):192–208. [PubMed] [Google Scholar]
  9. Jayaraman J., Cotman C., Mahler H. R., Sharp C. W. Biochemical correlates of respiratory deficiency. VII. Glucose repression. Arch Biochem Biophys. 1966 Sep 26;116(1):224–251. doi: 10.1016/0003-9861(66)90029-4. [DOI] [PubMed] [Google Scholar]
  10. LINDENMAYER A., ESTABROOK R. W. Low-temperature spectral studies on the biosynthesis of cytochromes in Baker's yeast. Arch Biochem Biophys. 1958 Nov;78(1):66–82. doi: 10.1016/0003-9861(58)90315-1. [DOI] [PubMed] [Google Scholar]
  11. LUBIN M., ENNIS H. L. ON THE ROLE OF INTRACELLULAR POTASSIUM IN PROTEIN SYNTHESIS. Biochim Biophys Acta. 1964 Apr 27;80:614–631. doi: 10.1016/0926-6550(64)90306-8. [DOI] [PubMed] [Google Scholar]
  12. PENNINGTON R. J. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase. Biochem J. 1961 Sep;80:649–654. doi: 10.1042/bj0800649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Polakis E. S., Bartley W. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J. 1965 Oct;97(1):284–297. doi: 10.1042/bj0970284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Polakis E. S., Bartley W., Meek G. A. Changes in the activities of respiratory enzymes during the aerobic growth of yeast on different carbon sources. Biochem J. 1965 Oct;97(1):298–302. doi: 10.1042/bj0970298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Polakis E. S., Bartley W., Meek G. A. Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem J. 1964 Feb;90(2):369–374. doi: 10.1042/bj0900369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. RACKER E. Crystalline alcohol dehydrogenase from baker's yeast. J Biol Chem. 1950 May;184(1):313–319. [PubMed] [Google Scholar]
  17. TUSTANOFF E. R., BARTLEY W. THE EFFECT OF GLUCOSE ON THE DEVELOPMENT OF RESPIRATION BY ANAEROBICALLY GROWN YEAST. Can J Biochem. 1964 May;42:651–665. doi: 10.1139/o64-078. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES