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. 1971 Feb;121(3):495–501. doi: 10.1042/bj1210495

Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system

K L Tao 1, T C Hall 1
PMCID: PMC1176597  PMID: 5119785

Abstract

1. Factors affecting aminoacyl-tRNA synthesis in vitro by cell-free preparations from bean leaves were investigated. 2. Evidence was obtained that optimum concentrations as well as correct ratios of Mg2+ and ATP are required for aminoacyl-tRNA synthesis in the bean-leaf system. 3. The results indicated that pH is a controlling factor having differential effects on the formation of individual aminoacyl-tRNA species. The possible micro-regulatory function of pH in protein synthesis in vivo is discussed with special reference to alanyl-tRNA formation. 4. Very low rates of alanine-stimulated pyrophosphate exchange were observed in the absence of tRNA. This observation is discussed relative to proposals about the mechanism of aminoacyl-tRNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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