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. 1971 Apr;122(3):333–345. doi: 10.1042/bj1220333

Protein phosphokinase reactions in mammalian testis. Stimulatory effects of adenosine 3′:5′-cyclic monophosphate on the phosphorylation of basic proteins

A H Reddi 1, L L Ewing 1,*, H G Williams-Ashman 1
PMCID: PMC1176783  PMID: 4330198

Abstract

The soluble fraction of rat testis homogenates is rich in enzymes catalysing the phosphorylation by ATP of many different proteins. The phosphorylation of certain histones, and also of a homogeneous basic protein from guinea-pig seminal-vesicle secretion, is markedly enhanced by 3′:5′-cyclic AMP. Various factors affecting these reactions as catalysed by partially purified testicular enzyme preparations are described, and their possible physiological significance is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURNETT G., KENNEDY E. P. The enzymatic phosphorylation of proteins. J Biol Chem. 1954 Dec;211(2):969–980. [PubMed] [Google Scholar]
  2. Corbin J. D., Krebs E. G. A cyclic AMP--stimulated protein kinase in adipose tissue. Biochem Biophys Res Commun. 1969 Jul 23;36(2):328–336. doi: 10.1016/0006-291x(69)90334-9. [DOI] [PubMed] [Google Scholar]
  3. Dixon G. H., Ingles C. J., Jergil B., Ling V., Marushige K. Protein transformations during differentiation of trout testis. Proc Can Cancer Conf. 1969;8:76–102. [PubMed] [Google Scholar]
  4. GOTTERER B., GINSBERG D., SCHULMAN T., BANKS J., WILLIAMS-ASHMAN H. G. Enzymatic coagulation of semen. Nature. 1955 Dec 24;176(4495):1209–1211. doi: 10.1038/1761209a0. [DOI] [PubMed] [Google Scholar]
  5. Gill G. N., Garren L. D. A cyclic-3',5'-adenosine monophosphate dependent protein kinase from the adrenal cortex: comparison with a cyclic AMP binding protein. Biochem Biophys Res Commun. 1970 May 11;39(3):335–343. doi: 10.1016/0006-291x(70)90581-4. [DOI] [PubMed] [Google Scholar]
  6. Griffin D. M., Szego C. M. Adenosine 3',5'-monophosphate stimulation of uterine amino acid uptake in vitro. Life Sci. 1968 Oct 15;7(20):1017–1023. doi: 10.1016/0024-3205(68)90137-9. [DOI] [PubMed] [Google Scholar]
  7. Hechter O., Yoshinaga K., Halkerston I. D., Birchall K. Estrogen-like anabolic effects of cyclic 3',5'-adenosine monophosphate and other nucleotides in isolated rat uterus. Arch Biochem Biophys. 1967 Nov;122(2):449–465. doi: 10.1016/0003-9861(67)90219-6. [DOI] [PubMed] [Google Scholar]
  8. Jard S., Bastide F. A cyclic AMP-dependent protein kinase from frog bladder epithelial cells. Biochem Biophys Res Commun. 1970 May 22;39(4):559–566. doi: 10.1016/0006-291x(70)90240-8. [DOI] [PubMed] [Google Scholar]
  9. Jergil B., Dixon G. H. Protamine kinase from rainbow trout testis. Partial purification and characterization. J Biol Chem. 1970 Jan 25;245(2):425–434. [PubMed] [Google Scholar]
  10. Johns E. W. Studies on histones. 7. Preparative methods for histone fractions from calf thymus. Biochem J. 1964 Jul;92(1):55–59. doi: 10.1042/bj0920055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. King R. J., Gordon J. The protein phosphokinase activity of the oestradiol-17beta-binding fraction from uterine nuclei and its stimulation by oestradiol-17beta. Biochem J. 1969 Oct;114(4):59P–60P. doi: 10.1042/bj1140059pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kissel J. H., Rosenfeld M. G., Chase L. R., O'Malley B. W. Response of chick oviduct adenyl cyclase to steroid hormones. Endocrinology. 1970 May;86(5):1019–1023. doi: 10.1210/endo-86-5-1019. [DOI] [PubMed] [Google Scholar]
  13. Kleinsmith L. J., Allfrey V. G., Mirsky A. E. Phosphoprotein metabolism in isolated lymphocyte nuclei. Proc Natl Acad Sci U S A. 1966 May;55(5):1182–1189. doi: 10.1073/pnas.55.5.1182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuehl F. A., Jr, Patanelli D. J., Tarnoff J., Humes J. L. Testicular adenyl cyclase: stimulation by the pituitary gonadotrophins. Biol Reprod. 1970 Feb;2(1):154–163. doi: 10.1095/biolreprod2.1.154. [DOI] [PubMed] [Google Scholar]
  15. Kuehl F. A., Jr, Patanelli D. J., Tarnoff J., Humes J. L. Testicular adenyl cyclase: stimulation by the pituitary gonadotrophins. Biol Reprod. 1970 Feb;2(1):154–163. doi: 10.1095/biolreprod2.1.154. [DOI] [PubMed] [Google Scholar]
  16. Kuo J. F., Greengard P. An adenosine 3',5'-monophosphate-dependent protein kinase from Escherichia coli. J Biol Chem. 1969 Jun 25;244(12):3417–3419. [PubMed] [Google Scholar]
  17. Kuo J. F., Greengard P. Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349–1355. doi: 10.1073/pnas.64.4.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Langan T. A. Action of adenosine 3',5'-monophosphate-dependent histone kinase in vivo. J Biol Chem. 1969 Oct 25;244(20):5763–5765. [PubMed] [Google Scholar]
  20. Langan T. A. Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate. Science. 1968 Nov 1;162(3853):579–580. doi: 10.1126/science.162.3853.579. [DOI] [PubMed] [Google Scholar]
  21. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  22. Meisler M. H., Langan T. A. Characterization of a phosphatase specific for phosphorylated histones and protamine. J Biol Chem. 1969 Sep 25;244(18):4961–4968. [PubMed] [Google Scholar]
  23. Miyamoto E., Kuo J. F., Greengard P. Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain. J Biol Chem. 1969 Dec 10;244(23):6395–6402. [PubMed] [Google Scholar]
  24. Murad F., Strauch B. S., Vaughan M. The effect of gonadotropins on testicular adenyl cyclase. Biochim Biophys Acta. 1969 May 6;177(3):591–598. doi: 10.1016/0304-4165(69)90324-9. [DOI] [PubMed] [Google Scholar]
  25. Murad F., Strauch B. S., Vaughan M. The effect of gonadotropins on testicular adenyl cyclase. Biochim Biophys Acta. 1969 May 6;177(3):591–598. doi: 10.1016/0304-4165(69)90324-9. [DOI] [PubMed] [Google Scholar]
  26. Notides A. C., Williams-Ashman H. G. The basic protein responsible for the clotting of guinea pig semen. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1991–1995. doi: 10.1073/pnas.58.5.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Ord M. G., Stocken L. A. Variations in the phosphate content and thiołdisulphide ratio of histones during the cell cycle. Studies with regenerating rat liver and sea urchins. Biochem J. 1968 Apr;107(3):403–410. doi: 10.1042/bj1070403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rosenfeld M. G., O'Malley B. W. Steroid hormones: effects on adenyl cyclase activity and adenosine 3',5'-momophosphate in target tissues. Science. 1970 Apr 10;168(3928):253–255. doi: 10.1126/science.168.3928.253. [DOI] [PubMed] [Google Scholar]
  29. Sharma S. K., Talwar G. P. Action of cyclic adenosine 3',5'-monophosphate in vitro on the uptake and incorporation of uridine into ribonucleic acid in ovariectomized rat uterus. J Biol Chem. 1970 Apr 10;245(7):1513–1519. [PubMed] [Google Scholar]
  30. Singhal R. L., Vijayvargiya R., Ling G. M. Cyclic adenosine monophosphate: andromimetic action on seminal vesicular enzymes. Science. 1970 Apr 10;168(3928):261–263. doi: 10.1126/science.168.3928.261. [DOI] [PubMed] [Google Scholar]
  31. Szego C. M., Davis J. S. Adenosine 3',5'-monophosphate in rat uterus: acute elevation by estrogen. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1711–1718. doi: 10.1073/pnas.58.4.1711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Walsh D. A., Perkins J. P., Krebs E. G. An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle. J Biol Chem. 1968 Jul 10;243(13):3763–3765. [PubMed] [Google Scholar]
  33. Yamamura H., Takeda M., Kumona, Nishizuka Y. Adenosine 3',5'-cyclic phosphate-dependent and independent histone kinases from rat liver. Biochem Biophys Res Commun. 1970 Aug 11;40(3):675–682. doi: 10.1016/0006-291x(70)90957-5. [DOI] [PubMed] [Google Scholar]

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