Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 Apr;122(3):347–351. doi: 10.1042/bj1220347

Thymidine kinase of rat liver. Activation by phospholipase C and subcellular distribution in the liver

F Stirpe *, M La Placa †
PMCID: PMC1176784  PMID: 4330199

Abstract

1. The thymidine kinase activity of rat liver is greatly enhanced on addition of phospholipase C to the assay mixture. 2. Most of the thymidine kinase activity of the liver is recovered in the mitochondrial and in the impure `nuclear' fractions. No activity was detected in purified nuclei prepared in high-density sucrose. 3. A substantial thymidine kinase activity could be detected, with the aid of phospholipase C, in all rat tissues examined.

Full text

PDF
347

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRESNICK E., KARJALA R. J. END-PRODUCT INHIBITION OF THYMIDINE KINASE ACTIVITY IN NORMAL AND LEUKEMIC HUMAN LEUKOCYTES. Cancer Res. 1964 Jun;24:841–846. [PubMed] [Google Scholar]
  2. BUCHER N. L. REGENERATION OF MAMMALIAN LIVER. Int Rev Cytol. 1963;15:245–300. doi: 10.1016/s0074-7696(08)61119-5. [DOI] [PubMed] [Google Scholar]
  3. Basilico C., Matsuya Y., Green H. Origin of the thymidine kinase induced by polyoma virus in productively infected cells. J Virol. 1969 Feb;3(2):140–145. doi: 10.1128/jvi.3.2.140-145.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baugnet-Mahieu L., Goutier R., Semal M. Studies on the intracellular distribution of thymidine phosphorylating kinase activities in regenerating rat liver. Eur J Biochem. 1968 Apr;4(3):323–328. doi: 10.1111/j.1432-1033.1968.tb00213.x. [DOI] [PubMed] [Google Scholar]
  5. Brent T. P., Butler J. A., Crathorn A. R. Variations in phosphokinase activities during the cell cycle in synchronous populations of HeLa cells. Nature. 1965 Jul 10;207(993):176–177. doi: 10.1038/207176a0. [DOI] [PubMed] [Google Scholar]
  6. Bresnick E., Thompson U. B. Properties of deoxythymidine kinase partially purified from animal tumors. J Biol Chem. 1965 Oct;240(10):3967–3974. [PubMed] [Google Scholar]
  7. CHAUVEAU J., MOULE Y., ROUILLER C. Isolation of pure and unaltered liver nuclei morphology and biochemical composition. Exp Cell Res. 1956 Aug;11(2):317–321. doi: 10.1016/0014-4827(56)90107-0. [DOI] [PubMed] [Google Scholar]
  8. Hatanaka M., Twiddy E., Gilden R. Electrophoresis of adenovirus-specified thymidine kinase. J Virol. 1969 Nov;4(5):801–803. doi: 10.1128/jvi.4.5.801-803.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klemperer H. G., Haynes G. R. Thymidine kinase in rat liver during development. Biochem J. 1968 Jul;108(4):541–546. doi: 10.1042/bj1080541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Morris H. P. Studies on the development, biochemistry, and biology of experimental hepatomas. Adv Cancer Res. 1965;9:227–302. doi: 10.1016/s0065-230x(08)60448-0. [DOI] [PubMed] [Google Scholar]
  12. Shiosaka T., Omura Y., Okuda H., Fujii S. Activation of thymidine kinase of adult rat liver by the culture filtrate of Clostridium perfringens. Biochim Biophys Acta. 1970 Apr 15;204(2):352–358. doi: 10.1016/0005-2787(70)90153-x. [DOI] [PubMed] [Google Scholar]
  13. Toide H., Okuda H., Shiosaka T., Fujii S. Purification of the factor involved in thymidine phosphorylation in adult rat liver. Biochim Biophys Acta. 1970 Oct 15;217(2):221–224. doi: 10.1016/0005-2787(70)90521-6. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES