Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 May;122(5):759–763. doi: 10.1042/bj1220759

Subcellular distribution of taurine and cysteinesulphinate decarboxylase in developing rat brain

H C Agrawal 1, A N Davison 1, L K Kaczmarek 1
PMCID: PMC1176845  PMID: 5129271

Abstract

The concentration of taurine and the activities of cysteinesulphinate decarboxylase and glutamate decarboxylase have been measured in rat brain. During development, taurine exhibited a decrease in concentration unrelated to the activity of cysteinesulphinate decarboxylase which increased during the same period. The distribution of taurine in subcellular fractions of adult and 7-day-old rat brain was typical of most amino acids, whereas half of the cysteinesulphinate decarboxylase activity was found in the nerve-ending cytoplasm. In anatomical distribution, taurine displayed great regional heterogeneity but both cysteinesulphinate decarboxylase and glutamate decarboxylase were more evenly distributed. Hypertaurinaemia was shown to have no effect on the entry of glycine into the brain or on its utilization in protein synthesis.

Full text

PDF
759

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AULUCK F. C., KOTHARI D. S. Radiation and sensation of pain. Nature. 1949 Nov 26;164(4178):923–923. doi: 10.1038/164923a0. [DOI] [PubMed] [Google Scholar]
  2. Agrawal H. C., Bone A. H., Davison A. N. Effect of phenylalanine on protein synthesis in the developing rat brain. Biochem J. 1970 Apr;117(2):325–331. doi: 10.1042/bj1170325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Agrawal H. C., Davis J. M., Himwich W. A. Maturational changes in amino acids in CNS of different mammalian species. Recent Adv Biol Psychiatry. 1968;10:258–265. doi: 10.1007/978-1-4684-9072-5_20. [DOI] [PubMed] [Google Scholar]
  4. Agrawal H. C., Davis J. M., Himwich W. A. Postnatal changes in free amino acid pool of rat brain. J Neurochem. 1966 Jul;13(7):607–615. doi: 10.1111/j.1471-4159.1966.tb11957.x. [DOI] [PubMed] [Google Scholar]
  5. Battistin L., Lajtha A. Regional distribution and movement of amino acids in the brain. J Neurol Sci. 1970 Mar;10(3):313–322. doi: 10.1016/0022-510x(70)90158-9. [DOI] [PubMed] [Google Scholar]
  6. Curtis D. R., Watkins J. C. The pharmacology of amino acids related to gamma-aminobutyric acid. Pharmacol Rev. 1965 Dec;17(4):347–391. [PubMed] [Google Scholar]
  7. DE ROBERTIS E., RODRIGUEZ DE LORES ARNAIZ G., SALGANICOFF L., PELLEGRINO DE IRALDI A., ZIEHER L. M. Isolation of synaptic vesicles and structural organization of the acetycholine system within brain nerve endings. J Neurochem. 1963 Apr;10:225–235. doi: 10.1111/j.1471-4159.1963.tb05038.x. [DOI] [PubMed] [Google Scholar]
  8. Fonnum F. The distribution of glutamate decarboxylase and aspartate transaminase in subcellular fractions of rat and guinea-pig brain. Biochem J. 1968 Jan;106(2):401–412. doi: 10.1042/bj1060401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gaitonde M. K., Short R. A. Quantitative determination of taurine by an o-phthalaldehyde-urea reaction. Analyst. 1971 Apr;96(141):274–280. doi: 10.1039/an9719600274. [DOI] [PubMed] [Google Scholar]
  10. Gaull G. E., Gaitonde M. K. A study of the sulphur amino acids of rat tissues. Biochem J. 1967 Jan;102(1):294–303. doi: 10.1042/bj1020294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HESS H. H., LEWIN E. MICROASSAY OF BIOCHEMICAL STRUCTURAL COMPONENTS IN NERVOUS TISSUES. II. METHODS FOR CEREBROSIDES, PROTEOLIPID PROTEINS AND RESIDUE PROTEINS. J Neurochem. 1965 Mar;12:205–211. doi: 10.1111/j.1471-4159.1965.tb06756.x. [DOI] [PubMed] [Google Scholar]
  12. JACOBSEN J. G., THOMAS L. L., SMITH L. H., Jr PROPERTIES AND DISTRIBUTION OF MAMMALIAN L-CYSTEINE SULFINATE CARBOXY-LYASES. Biochim Biophys Acta. 1964 Apr 6;85:103–116. doi: 10.1016/0926-6569(64)90171-3. [DOI] [PubMed] [Google Scholar]
  13. Krnjević K. Glutamate and gamma-aminobutyric acid in brain. Nature. 1970 Oct 10;228(5267):119–124. doi: 10.1038/228119a0. [DOI] [PubMed] [Google Scholar]
  14. Kuriyama K., Roberts E., Vos J. Some characteristics of binding of gamma-aminobutyric acid and acetylcholine to a synaptic vesicle fraction form mouse brain. Brain Res. 1968 Jul;9(2):231–252. doi: 10.1016/0006-8993(68)90232-1. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Mangan J. L., Whittaker V. P. The distribution of free amino acids in subcellular fractions of guinea-pig brain. Biochem J. 1966 Jan;98(1):128–137. doi: 10.1042/bj0980128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peck E. J., Jr, Awapara J. Formation of taurine and isethionic acid in rat brain. Biochim Biophys Acta. 1967 Aug 29;141(3):499–506. doi: 10.1016/0304-4165(67)90178-x. [DOI] [PubMed] [Google Scholar]
  18. RYALL R. W. THE SUBCELLULAR DISTRIBUTIONS OF ACETYLCHOLINE, SUBSTANCE P, 5-HYDROXYTRYPTAMINE, GAMMA-AMINOBUTYRIC ACID AND GLUTAMIC ACID IN BRAIN HOMOGENATES. J Neurochem. 1964 Mar;11:131–145. doi: 10.1111/j.1471-4159.1964.tb06124.x. [DOI] [PubMed] [Google Scholar]
  19. Ramírez de Guglielmone, Gómez C. J. Influence of neonatal hypothyroidism on amino acids in developing rat brain. J Neurochem. 1966 Oct;13(10):1017–1025. doi: 10.1111/j.1471-4159.1966.tb10300.x. [DOI] [PubMed] [Google Scholar]
  20. SALGANICOFF L., DEROBERTIS E. SUBCELLULAR DISTRIBUTION OF THE ENZYMES OF THE GLUTAMIC ACID, GLUTAMINE AND GAMMA-AMINOBUTYRIC ACID CYCLES IN RAT BRAIN. J Neurochem. 1965 Apr;12:287–309. doi: 10.1111/j.1471-4159.1965.tb06766.x. [DOI] [PubMed] [Google Scholar]
  21. Sims K. L., Pitts F. N., Jr Brain glutamate decarboxylase: changes in the developing rat brain. J Neurochem. 1970 Nov;17(11):1607–1612. doi: 10.1111/j.1471-4159.1970.tb03731.x. [DOI] [PubMed] [Google Scholar]
  22. THOMAS J. J., GOODMAN H. O., KING J. S., Jr, WAINER A. TAURINE EXCRETION AND INTELLIGENCE IN MONGOLISM. Proc Soc Exp Biol Med. 1965 Jul;119:832–833. doi: 10.3181/00379727-119-30313. [DOI] [PubMed] [Google Scholar]
  23. WELTY J. D., READ W. O. STUDIES ON SOME CARDIAC EFFECT OF TAURINE. J Pharmacol Exp Ther. 1964 Apr;144:110–115. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES