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. 1971 Jun;123(1):35–39. doi: 10.1042/bj1230035

Phosphoenolpyruvate carboxykinase of kidney. Subcellular distribution and response to acid–base changes

H Flores 1, G A O Alleyne 1
PMCID: PMC1176897  PMID: 5128664

Abstract

1. A method for the assay of phosphoenolpyruvate carboxykinase is presented, based on the enzymic determination of the phosphoenolpyruvate produced by the enzyme reaction. 2. The subcellular distribution of phosphoenolpyruvate carboxykinase in the kidney of several animal species resembled the distribution in the liver. 3. The rise in enzyme activity in the kidney cortex of rats made acidotic by feeding with ammonium chloride was not prevented by administration of ethionine or actinomycin. 4. The possibility is suggested that in the kidney acidosis causes activation of an inactive form of the enzyme already present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alleyne G. A. Concentrations of metabolic intermediates in kidneys of rats with metabolic acidosis. Nature. 1968 Mar 2;217(5131):847–848. doi: 10.1038/217847a0. [DOI] [PubMed] [Google Scholar]
  2. Alleyne G. A. Renal metabolic response to acid-base changes. II. The early effects of metabolic acidosis on renal metabolism in the rat. J Clin Invest. 1970 May;49(5):943–951. doi: 10.1172/JCI106314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alleyne G. A., Scullard G. H. Renal metabolic response to acid base changes. I. Enzymatic control of ammoniagenesis in the rat. J Clin Invest. 1969 Feb;48(2):364–370. doi: 10.1172/JCI105993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ballard F. J., Hanson R. W. Purification of phosphoenolpyruvate carboxykinase from the cytosol fraction of rat liver and the immunochemical demonstration of differences between this enzyme and the mitochondrial phosphoenolpyruvate carboxykinase. J Biol Chem. 1969 Oct 25;244(20):5625–5630. [PubMed] [Google Scholar]
  5. Gevers W. The regulation of phosphoenolpyruvate synthesis in pigeon liver. Biochem J. 1967 Apr;103(1):141–152. doi: 10.1042/bj1030141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodman A. D., Fuisz R. E., Cahill G. F., Jr Renal gluconeogenesis in acidosis, alkalosis, and potassium deficiency: its possible role in regulation of renal ammonia production. J Clin Invest. 1966 Apr;45(4):612–619. doi: 10.1172/JCI105375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goorno W. E., Rector F. C., Jr, Seldin D. W. Relation of renal gluconeogenesis to ammonia production in the dog and rat. Am J Physiol. 1967 Oct;213(4):969–974. doi: 10.1152/ajplegacy.1967.213.4.969. [DOI] [PubMed] [Google Scholar]
  8. Kamm D. E., Fuisz R. E., Goodman A. D., Cahill G. F., Jr Acid-base alterations and renal gluconeogenesis: effect of pH, bicarbonate concentration, and PCO2. J Clin Invest. 1967 Jul;46(7):1172–1177. doi: 10.1172/JCI105610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. NORDLIE R. C., LARDY H. A. Mammalian liver phosphoneolpyruvate carboxykinase activities. J Biol Chem. 1963 Jul;238:2259–2263. [PubMed] [Google Scholar]
  11. SHRAGO E., LARDY H. A., NORDLIE R. C., FOSTER D. O. METABOLIC AND HORMONAL CONTROL OF PHOSPHOENOLPYRUVATE CARBOXYKINASE AND MALIC ENZYME IN RAT LIVER. J Biol Chem. 1963 Oct;238:3188–3192. [PubMed] [Google Scholar]
  12. Seubert W., Huth W. On the mechanism of gluconeogenesis and its regulation. II. The mechanism of gluconeogenesis from pyruvate and fumarate. Biochem Z. 1965 Nov 15;343(2):176–191. [PubMed] [Google Scholar]

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