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. 1971 Jun;123(1):57–67. doi: 10.1042/bj1230057

Amino acid sequence of the encephalitogenic basic protein from human myelin

P R Carnegie 1,*
PMCID: PMC1176899  PMID: 4108501

Abstract

Myelin from the central nervous system contains an unusual basic protein, which can induce experimental autoimmune encephalomyelitis. The basic protein from human brain was digested with trypsin and other enzymes and the sequence of the 170 amino acids was determined. The localization of the encephalitogenic determinants was described. Possible roles for the protein in the structure and function of myelin are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBLER R. P. THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551. Biochem J. 1963 Nov;89:349–378. doi: 10.1042/bj0890349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Agrawal H. C., Banik N. L., Bone A. H., Davison A. N., Mitchell R. F., Spohn M. The identity of a myelin-like fraction isolated from developing brain. Biochem J. 1970 Dec;120(3):635–642. doi: 10.1042/bj1200635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ambler R. P., Meadway R. J. The use of thermolysin in amino acid sequence determination. Biochem J. 1968 Aug;108(5):893–895. doi: 10.1042/bj1080893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baldwin G. S., Carnegie P. R. Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin. Biochem J. 1971 Jun;123(1):69–74. doi: 10.1042/bj1230069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baldwin G. S., Carnegie P. R. Specific enzymic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin. Science. 1971 Feb 12;171(3971):579–581. doi: 10.1126/science.171.3971.579. [DOI] [PubMed] [Google Scholar]
  6. Bencina B., Carnegie P. R., McPherson T. A., Robson G. Encephalitogenic basic protein from sciatic nerve. FEBS Lett. 1969 Jul;4(1):9–12. doi: 10.1016/0014-5793(69)80181-x. [DOI] [PubMed] [Google Scholar]
  7. Blow D. M., Birktoft J. J., Hartley B. S. Role of a buried acid group in the mechanism of action of chymotrypsin. Nature. 1969 Jan 25;221(5178):337–340. doi: 10.1038/221337a0. [DOI] [PubMed] [Google Scholar]
  8. Bunge R. P. Glial cells and the central myelin sheath. Physiol Rev. 1968 Jan;48(1):197–251. doi: 10.1152/physrev.1968.48.1.197. [DOI] [PubMed] [Google Scholar]
  9. CASPARY E. A., FIELD E. J. AN ENCEPHALITOGENIC PROTEIN OF HUMAN ORIGIN: SOME CHEMICAL AND BIOLOGICAL PROPERTIES. Ann N Y Acad Sci. 1965 Mar 31;122:182–198. doi: 10.1111/j.1749-6632.1965.tb20202.x. [DOI] [PubMed] [Google Scholar]
  10. Carnegie P. R., Bencina B., Lamoureux G. Experimental allergic encephalomyelitis. Isolation of basic proteins and polypeptides from central nervous tissue. Biochem J. 1967 Nov;105(2):559–568. doi: 10.1042/bj1050559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Carnegie P. R. Digestion of an arg-pro bond by trypsin in the ecephalitogenic basic protein of human myelin. Nature. 1969 Aug 30;223(5209):958–959. doi: 10.1038/223958a0. [DOI] [PubMed] [Google Scholar]
  12. Carnegie P. R., Lumsden C. E. Fractionation of encephalitogenic polypeptides from bovine spinal cord by gel filtration in phenol--acetic acid--water. Immunology. 1967 Feb;12(2):133–145. [PMC free article] [PubMed] [Google Scholar]
  13. Carnegie P. R., McPherson T. A., Robson G. S. Experimental autoimmune encephalomyelitis. Digestion of basic protein of human myelin with cyanogen bromide and trypsin. Immunology. 1970 Jul;19(1):55–63. [PMC free article] [PubMed] [Google Scholar]
  14. Carnegie P. R. N-terminal sequence of an encephalitogenic protein form human myelin. Biochem J. 1969 Jan;111(2):240–242. doi: 10.1042/bj1110240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Carnegie P. R. Properties, structure and possible neuroreceptor role of the encephalitogenic protein of human brain. Nature. 1971 Jan 1;229(5279):25–28. doi: 10.1038/229025a0. [DOI] [PubMed] [Google Scholar]
  16. Chao L. P., Einstein E. R. Physical properties of the bovine encephalitogenic protein; molecular weight and conformation. J Neurochem. 1970 Aug;17(8):1121–1132. doi: 10.1111/j.1471-4159.1970.tb03360.x. [DOI] [PubMed] [Google Scholar]
  17. Dickinson J. P., Jones K. M., Aparicio S. R., Lumsden C. E. Localization of encephalitogenic basic protein in the intraperiod line of lamellar myelin. Nature. 1970 Sep 12;227(5263):1133–1134. doi: 10.1038/2271133a0. [DOI] [PubMed] [Google Scholar]
  18. Eng L. F., Chao F. C., Gerstl B., Pratt D., Tavaststjerna M. G. The maturation of human white matter myelin. Fractionation of the myelin membrane proteins. Biochemistry. 1968 Dec;7(12):4455–4465. doi: 10.1021/bi00852a042. [DOI] [PubMed] [Google Scholar]
  19. Eylar E. H., Hashim G. A. Allergic encephalomyelitis: the structure of encephalitogenic determinant. Proc Natl Acad Sci U S A. 1968 Oct;61(2):644–650. doi: 10.1073/pnas.61.2.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Field E. J., Caspary E. A. Lymphocyte sensitisation: an in-vitro test for cancer? Lancet. 1970 Dec 26;2(7687):1337–1341. doi: 10.1016/s0140-6736(70)92361-5. [DOI] [PubMed] [Google Scholar]
  21. Field E. J., Raine C. S., Hughes D. Failure to induce myelin sheath formation around artificial fibres with a note on the toxicity of polyester fibres for nervous tissue in vitrol. J Neurol Sci. 1969 Jan-Feb;8(1):129–141. doi: 10.1016/0022-510x(69)90046-x. [DOI] [PubMed] [Google Scholar]
  22. Hallpike J. F., Adams C. W., Bayliss O. B. Histochemistry of myelin. XI. Loss of basic protein in early myelin breakdown and multiple sclerosis plaques. Histochem J. 1970 Jul;2(4):323–328. doi: 10.1007/BF01005000. [DOI] [PubMed] [Google Scholar]
  23. Hartley B. S. Strategy and tactics in protein chemistry. Biochem J. 1970 Oct;119(5):805–822. doi: 10.1042/bj1190805f. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Hashim G. A., Eylar E. H. The structure of the terminal regions of the encephalitogenic basic protein from bovine myelin. Arch Biochem Biophys. 1969 Dec;135(1):324–333. doi: 10.1016/0003-9861(69)90546-3. [DOI] [PubMed] [Google Scholar]
  25. Kibler R. F., Shapira R., McKneally S., Jenkins J., Selden P., Chou F. Encephalitogenic protein: structure. Science. 1969 May 2;164(3879):577–580. doi: 10.1126/science.164.3879.577. [DOI] [PubMed] [Google Scholar]
  26. Lennon V. A., Wilks A. V., Carnegie P. R. Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin. J Immunol. 1970 Nov;105(5):1223–1230. [PubMed] [Google Scholar]
  27. Lumsden C. E., Robertson D. M., Blight R. Chemical studies on experimental allergic encephalomyelitis. Peptide as the common denominator in all encephalitogenic 'antigens'. J Neurochem. 1966 Mar;13(3):127–162. doi: 10.1111/j.1471-4159.1966.tb07507.x. [DOI] [PubMed] [Google Scholar]
  28. Martenson R. E., Deibler G. E., Kies M. W. Myelin basic proteins of the rat central nervous system. Purification, encephalitogenic properties, and amino acid compositions. Biochim Biophys Acta. 1970 Feb 17;200(2):353–362. doi: 10.1016/0005-2795(70)90177-7. [DOI] [PubMed] [Google Scholar]
  29. Milstein C. A simple procedure for the fractionation of the tryptic peptides of the C-terminal half of immunoglobulin lambda-chains. Biochem J. 1968 Dec;110(4):652–654. doi: 10.1042/bj1100652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  31. Oshiro Y., Eylar E. H. Allergic encephalomyelitis: a comparison of the encephalitogenic A1 protein from human and bovine brain. Arch Biochem Biophys. 1970 Jun;138(2):606–613. doi: 10.1016/0003-9861(70)90387-5. [DOI] [PubMed] [Google Scholar]
  32. Palmer F. B., Dawson R. M. The isolation and properties of experimental allergic encephalitogenic protein. Biochem J. 1969 Mar;111(5):629–636. doi: 10.1042/bj1110629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Pink J. R., Buttery S. H., De Vries G. M., Milstein C. Human immunoglobulin subclasses. Partial amino acid sequence of the constant region of a gamma 4 chain. Biochem J. 1970 Mar;117(1):33–47. doi: 10.1042/bj1170033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Piszkiewicz D., Landon M., Smith E. L. Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations. Biochem Biophys Res Commun. 1970 Sep 10;40(5):1173–1178. doi: 10.1016/0006-291x(70)90918-6. [DOI] [PubMed] [Google Scholar]
  35. Swaney J. B., Klotz I. M. Amino acid sequence adjoining the lone tryptophan of human serum albumin. A binding site of the protein. Biochemistry. 1970 Jun 23;9(13):2570–2574. doi: 10.1021/bi00815a003. [DOI] [PubMed] [Google Scholar]
  36. WALEY S. G., WATSON J. The action of trypsin on polylysine. Biochem J. 1953 Sep;55(2):328–337. doi: 10.1042/bj0550328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. WANG S. S., CARPENTER F. H. A COMPOSITIONAL ASSAY FOR INSULIN APPLIED TO A SEARCH FOR "PROINSULIN". J Biol Chem. 1965 Apr;240:1619–1625. [PubMed] [Google Scholar]
  38. Westall F. C., Robinson A. B., Caccam J., Jackson J., Ylar E. H. Essential chemical requirements for induction of allergic encephalomyelitis. Nature. 1971 Jan 1;229(5279):22–24. doi: 10.1038/229022a0. [DOI] [PubMed] [Google Scholar]

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