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. 1971 Jun;123(1):69–74. doi: 10.1042/bj1230069

Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin

G S Baldwin 1, P R Carnegie 1,*
PMCID: PMC1176900  PMID: 5128665

Abstract

Two methylated derivatives of arginine were isolated from the encephalitogenic protein of myelin from the central nervous system. Evidence is presented for the proposed structures, ω-NN′-dimethylarginine and ω-N-monomethylarginine. In the encephalitogenic protein from human brain the proportion 1:6:10 for arginine:monomethylarginine:dimethylarginine residues was found to occur at position 107. Possible roles for the methylated arginine in conformational changes and altered ion-exchange behaviour are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBLER R. P., REES M. W. Epsilon-N-Methyl-lysine in bacterial flagellar protein. Nature. 1959 Jul 4;184:56–57. doi: 10.1038/184056b0. [DOI] [PubMed] [Google Scholar]
  2. Asatoor A. M., Armstrong M. D. 3-methylhistidine, a component of actin. Biochem Biophys Res Commun. 1967 Jan 23;26(2):168–174. doi: 10.1016/0006-291x(67)90229-x. [DOI] [PubMed] [Google Scholar]
  3. Baldwin G. S., Carnegie P. R. Specific enzymic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin. Science. 1971 Feb 12;171(3971):579–581. doi: 10.1126/science.171.3971.579. [DOI] [PubMed] [Google Scholar]
  4. CARNEGIE P. R., SYNGE R. L. Filter-paper ionophoresis of cupric complexes of neutral amino acids and oligopeptides. Biochem J. 1961 Apr;78:692–696. doi: 10.1042/bj0780692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carnegie P. R. Amino acid sequence of the encephalitogenic basic protein from human myelin. Biochem J. 1971 Jun;123(1):57–67. doi: 10.1042/bj1230057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carnegie P. R., Bencina B., Lamoureux G. Experimental allergic encephalomyelitis. Isolation of basic proteins and polypeptides from central nervous tissue. Biochem J. 1967 Nov;105(2):559–568. doi: 10.1042/bj1050559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
  8. Eylar E. H. Amino acid sequence of the basic protein of the myelin membrane. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1425–1431. doi: 10.1073/pnas.67.3.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hatano H., Sumizu K., Rokushika S., Murakami F. Automatic liquid chromatography of primary mono- and diamines on a cation-exchange resin. Anal Biochem. 1970 Jun;35(2):377–383. doi: 10.1016/0003-2697(70)90198-3. [DOI] [PubMed] [Google Scholar]
  10. Kaye A. M., Sheratzky D. Methylation of protein (histone) in vitro: enzymic activity from the soluble fraction of rat organs. Biochim Biophys Acta. 1969 Oct 22;190(2):527–538. doi: 10.1016/0005-2787(69)90101-4. [DOI] [PubMed] [Google Scholar]
  11. Martenson R. E., Deibler G. E., Kies M. W. Microheterogeneity of guinea pig myelin basic protein. J Biol Chem. 1969 Aug 25;244(16):4261–4267. [PubMed] [Google Scholar]
  12. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  13. Paik W. K., Kim S. Omega-N-methylarginine in protein. J Biol Chem. 1970 Jan 10;245(1):88–92. [PubMed] [Google Scholar]
  14. Paik W. K., Kim S. Protein methylase I. Purification and properties of the enzyme. J Biol Chem. 1968 May 10;243(9):2108–2114. [PubMed] [Google Scholar]
  15. Paik W. K., Kim S. Protein methylation in rat brain in vitro. J Neurochem. 1969 Aug;16(8):1257–1261. doi: 10.1111/j.1471-4159.1969.tb05974.x. [DOI] [PubMed] [Google Scholar]

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