Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1971 Jul;123(4):601–605. doi: 10.1042/bj1230601

Sporulation in Bacillus subtilis. The appearance of sulpholactic acid as a marker event for sporulation

D A Wood 1
PMCID: PMC1177000  PMID: 5001733

Abstract

1. The synthesis of sulpholactic acid in sporulating cultures of Bacillus subtilis was studied. 2. Sulpholactic acid was first detected about 4h after the initiation of sporulation and 1h before refractility. The rate of synthesis paralleled that of the other events of sporulation examined. 3. Sulpholactic acid accounted for 1.7% of the material of the spore. 4. Because the addition of chloramphenicol in the earlier stages of sporulation inhibited formation of the compound, it is likely that the enzymes concerned are synthesized de novo during sporulation. 5. In asporogenous mutants only those blocked at a late stage and showing partial refractility were able to produce sulpholactic acid. This correlation makes sulpholactic acid a useful marker event in sporulation.

Full text

PDF
601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonsen P. P., Spudich J. A., Nelson D. L., Kornberg A. Biochemical studies of bacterial sporulation and germination. XII. A sulfonic acid as a major sulfur compound of Bacillus subtilis spores. J Bacteriol. 1969 Apr;98(1):62–68. doi: 10.1128/jb.98.1.62-68.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dawes I. W., Kay D., Mandelstam J. Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events. J Gen Microbiol. 1969 May;56(2):171–179. doi: 10.1099/00221287-56-2-171. [DOI] [PubMed] [Google Scholar]
  3. Kay D., Warren S. C. Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968 Oct;109(5):819–824. doi: 10.1042/bj1090819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Mandelstam J., Waites W. M. Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968 Oct;109(5):793–801. doi: 10.1042/bj1090793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. POWELL J. F. Isolation of dipicolinic acid (pyridine-2:6-dicarboxylic acid) from spores of Bacillus megatherium. Biochem J. 1953 May;54(2):210–211. doi: 10.1042/bj0540210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SZULMAJSTER J. BIOCHIMIE DE LA SPOROG'EN'ESE CHEZ B. SUBTILIS. Bull Soc Chim Biol (Paris) 1964;46:443–481. [PubMed] [Google Scholar]
  8. Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Waites W. M., Kay D., Dawes I. W., Wood D. A., Warren S. C., Mandelstam J. Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. Biochem J. 1970 Jul;118(4):667–676. doi: 10.1042/bj1180667. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES