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. 1971 Jul;123(4):643–648. doi: 10.1042/bj1230643

The separation of intracellular serum albumin from rat liver

J D Judah 1, Marion R Nicholls 1
PMCID: PMC1177006  PMID: 4942452

Abstract

1. Antibody precipitation of serum albumin from rat liver extracts yields impure preparations of the protein. 2. When rat liver is labelled with l-[1-14C]leucine, antibody precipitation of albumin leads to material that is contaminated with a protein or proteins of very high specific radioactivity. Only 10–25% of the radioactivity of the antibody precipitate is associated with serum albumin. 3. A chromatographic procedure is described that can be used to separate radiochemically pure serum albumin from antibody precipitates obtained from extracts of rat liver. 4. Extracellular albumin secreted by liver slices yields a precipitate with antibody which contains much less radioactive impurity. About 70–90% of the radioactivity is associated with serum albumin. Serum albumin separated by antibody precipitation from rat serum labelled in vivo was not contaminated with the radiochemical impurities associated with intracellular albumin. 5. A simple method is described of obtaining the content of serum albumin in rat liver extracts by the technique of isotope dilution and ion-exchange chromatography.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL P. N., STONE N. E. The synthesis of serum albumin and tissue proteins in slices of rat liver and liver tumour. Biochem J. 1957 May;66(1):19–31. doi: 10.1042/bj0660019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DEBRO J. R., TARVER H., KORNER A. The determination of serum albumin and globulin by a new method. J Lab Clin Med. 1957 Nov;50(5):728–732. [PubMed] [Google Scholar]
  3. GORDON A. H., HUMPHREY J. H. Measurement of intracellular albumin in rat liver. Biochem J. 1961 Mar;78:551–556. doi: 10.1042/bj0780551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Judah J. D., Nicholls M. R. Role of liver-cell potassium ions in secretion of serum albumin and lipoproteins. Biochem J. 1970 Feb;116(4):663–669. doi: 10.1042/bj1160663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KOEN A. L., SHAW C. R. A PREPARATIVE METHOD EMPLOYING STARCH GEL ELECTROPHORESIS AND ELECTRODIALYSIS. Anal Biochem. 1964 Dec;9:495–498. doi: 10.1016/0003-2697(64)90215-5. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. PETERS T., Jr The biosynthesis of rat serum albumin. II. Intracellular phenomena in the secretion of newly formed albumin. J Biol Chem. 1962 Apr;237:1186–1189. [PubMed] [Google Scholar]
  8. Schreiber G., Rotermund H. M., Maeno H., Weigand K., Lesch R. The proption of the incorporation of leucine into albumin to that into totaprotein in rat liver and hepatoma Morris 5123 TC. Eur J Biochem. 1969 Sep;10(2):355–361. doi: 10.1111/j.1432-1033.1969.tb00698.x. [DOI] [PubMed] [Google Scholar]

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