Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Mar;131(3):619–621. doi: 10.1042/bj1310619

Selective extraction of polymers from cell walls of Gram-positive bacteria (Short Communication)

Jiri G Pavlik 1, Howard J Rogers 1
PMCID: PMC1177511  PMID: 4198361

Abstract

Brief heating of Bacillus Licheniformis cell walls at 100°C in aqueous buffers of pH3.0–4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100°C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.

Full text

PDF
619

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. Archibald A. R., Coapes H. E., Stafford G. H. The action of dilute alkali on bacterial cell walls. Biochem J. 1969 Aug;113(5):899–900. doi: 10.1042/bj1130899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duckworth M., Archibald A. R., Baddiley J. The location of N-acetylgalactosamine in the walls of Bacillus subtilis 168. Biochem J. 1972 Dec;130(3):691–696. doi: 10.1042/bj1300691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grant W. D., Wicken A. J. Muramic acid phosphate and the linkage of teichoic acid to peptidoglycan in Bacillus stearothermophilus cell walls. Biochem Biophys Res Commun. 1968 Jul 26;32(2):122–128. doi: 10.1016/0006-291x(68)90356-2. [DOI] [PubMed] [Google Scholar]
  5. Hall E. A., Knox K. W. Properties of the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei. Biochem J. 1965 Aug;96(2):310–318. doi: 10.1042/bj0960310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hase S., Matsushima Y. Structural studies on a glucose containing polysaccharide obtained from Micrococcus lysodeikticus cell walls. I. J Biochem. 1970 Nov;68(5):723–730. doi: 10.1093/oxfordjournals.jbchem.a129406. [DOI] [PubMed] [Google Scholar]
  7. Hase S., Matsushima Y. Structural studies on a glucose-containing polysaccharide obtained from Micrococcus lysodeikticus cell walls. II. Determination of the linkage points on N-acetyl-mannosaminuronic acid and its configuration. J Biochem. 1971 Mar;69(3):559–565. [PubMed] [Google Scholar]
  8. Hughes R. C., Pavlik J. G., Rogers H. J., Tanner P. J. Organization of polymers in the cell walls of some bacilli. Nature. 1968 Aug 10;219(5154):642–644. doi: 10.1038/219642a0. [DOI] [PubMed] [Google Scholar]
  9. Hughes R. C., Tanner P. J. The action of dilute alkali on some bacterial cell walls. Biochem Biophys Res Commun. 1968 Oct 10;33(1):22–28. doi: 10.1016/0006-291x(68)90248-9. [DOI] [PubMed] [Google Scholar]
  10. Hughes R. C. The cell wall of Bacillus licheniformis N.C.T.C. 6346. Linkage between the teichuronic acid and mucopeptide components. Biochem J. 1970 Apr;117(3):431–439. doi: 10.1042/bj1170431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hughes R. C., Thurman P. F. Some structural features of the teichuronic acid of Bacillus licheniformis N.C.T.C. 6346 cell walls. Biochem J. 1970 Apr;117(3):441–449. doi: 10.1042/bj1170441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JANCZURA E., PERKINS H. R., ROGERS H. J. Teichuronic acid: a mucopolysaccharide present in wall preparations from vegetative cells of Bacillus subtilis. Biochem J. 1961 Jul;80:82–93. doi: 10.1042/bj0800082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MOKRASCH L. C. Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. J Biol Chem. 1954 May;208(1):55–59. [PubMed] [Google Scholar]
  14. PERKINS H. R. A polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus. Biochem J. 1963 Mar;86:475–483. doi: 10.1042/bj0860475. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES