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. 1973 Apr;131(4):689–697. doi: 10.1042/bj1310689

The oestrogen-stimulated synthesis of heterogeneous nuclear ribonucleic acid in the uterus of immature rats

J T Knowler 1, R M S Smellie 1
PMCID: PMC1177528  PMID: 4722448

Abstract

An early response to the administration of oestradiol-17β to immature rats is the synthesis of uterine RNA of very high-molecular-weight. This RNA is shown to be heterogeneous nuclear RNA. Increased precursor incorporation into the heterogeneous nRNA is not confined to entities of precise molecular weight but appears to involve much of the size range of the species. These findings are discussed with respect to the mode of action of oestradiol.

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Selected References

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  1. AIZAWA Y., MUELLER G. C. The effect in vivo and in vitro of estrogens on lipid synthesis in the rat uterus. J Biol Chem. 1961 Feb;236:381–386. [PubMed] [Google Scholar]
  2. Ab G., Malt R. A. Metabolism of ribosomal precursor ribonucleic acid in kidney. J Cell Biol. 1970 Aug;46(2):362–369. doi: 10.1083/jcb.46.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barker K. L. Estrogen-induced synthesis of histones and a specific nonhistone protein in the uterus. Biochemistry. 1971 Jan 19;10(2):284–291. doi: 10.1021/bi00778a014. [DOI] [PubMed] [Google Scholar]
  4. Barry J., Gorski J. Uterine ribonucleic acid polymerase. Effect of estrogen on nucleotide incorporation into 3' chain termini. Biochemistry. 1971 Jun 8;10(12):2384–2390. doi: 10.1021/bi00788a032. [DOI] [PubMed] [Google Scholar]
  5. Church R. B., McCarthy B. J. Unstable nuclear RNA synthesis following estrogen stimulation. Biochim Biophys Acta. 1970 Jan 21;199(1):103–114. doi: 10.1016/0005-2787(70)90699-4. [DOI] [PubMed] [Google Scholar]
  6. Darnell J. E., Philipson L., Wall R., Adesnik M. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971 Oct 29;174(4008):507–510. doi: 10.1126/science.174.4008.507. [DOI] [PubMed] [Google Scholar]
  7. DeAngelo A. B., Gorski J. Role of RNA synthesis in the estrogen induction of a specific uterine protein. Proc Natl Acad Sci U S A. 1970 Jul;66(3):693–700. doi: 10.1073/pnas.66.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIRARD M., LATHAM H., PENMAN S., DARNELL J. E. ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM. J Mol Biol. 1965 Feb;11:187–201. doi: 10.1016/s0022-2836(65)80050-x. [DOI] [PubMed] [Google Scholar]
  9. GORSKI J., NICOLETTE J. A. EARLY ESTROGEN EFFECTS ON NEWLY SYNTHESIZED RNA AND PHOSPHOLIPID IN SUBCELLULAR FRACTIONS OF RAT UTERI. Arch Biochem Biophys. 1963 Dec;103:418–423. doi: 10.1016/0003-9861(63)90431-4. [DOI] [PubMed] [Google Scholar]
  10. Glasser S. R., Chytil F., Spelsberg T. C. Early effects of oestradiol-17 on the chromatin and activity of the deoxyribonucleic acid-dependent ribonucleic acid polymerases (I and II) of the rat uterus. Biochem J. 1972 Dec;130(4):947–957. doi: 10.1042/bj1300947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hamilton T. H., Widnell C. C., Tata J. R. Sequential stimulations by oestrogen of nuclear RNA synthesis and DNA-dependent RNA polymerase activities in rat uterus. Biochim Biophys Acta. 1965 Sep 6;108(1):168–172. doi: 10.1016/0005-2787(65)90129-2. [DOI] [PubMed] [Google Scholar]
  12. Higashi K., Narayanan K. S., Adams H. R., Busch H. Utilization of the citric acid procedure and zonal ultracentrifugation for mass isolation of nuclear RNA from Walker 256 carcinosarcoma. Cancer Res. 1966 Jul;26(7):1582–1590. [PubMed] [Google Scholar]
  13. Katz L., Penman S. The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells. Biochem Biophys Res Commun. 1966 May 25;23(4):557–560. doi: 10.1016/0006-291x(66)90765-0. [DOI] [PubMed] [Google Scholar]
  14. Knowler J. T., Smellie R. M. The synthesis of ribonucleic acid in immature rat uterus responding to oestradiol-17 beta. Biochem J. 1971 Nov;125(2):605–614. doi: 10.1042/bj1250605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marcaud L., Portier M. M., Kourilsky P., Barrell B. G., Gros F. A low molecular weight species of RNA synthesized early after induction of the prophage lambda. J Mol Biol. 1971 Apr 28;57(2):247–261. doi: 10.1016/0022-2836(71)90344-5. [DOI] [PubMed] [Google Scholar]
  16. Notides A., Gorski J. Estrogen-induced synthesis of a specific uterine protein. Proc Natl Acad Sci U S A. 1966 Jul;56(1):230–235. doi: 10.1073/pnas.56.1.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  18. Penman S., Smith I., Holtzman E. Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus. Science. 1966 Nov 11;154(3750):786–789. doi: 10.1126/science.154.3750.786. [DOI] [PubMed] [Google Scholar]
  19. Rosenfeld G. C., Comstock J. P., Means A. R., O'Malley B. W. Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system. Biochem Biophys Res Commun. 1972 Feb 25;46(4):1695–1703. doi: 10.1016/0006-291x(72)90805-4. [DOI] [PubMed] [Google Scholar]
  20. SEBRING E. D., SALZMAN N. P. AN IMPROVED PROCEDURE FOR MEASURING THE DISTRIBUTION OF P32O4--AMONG THE NUCLEOTIDES OF RIBONUCLEIC ACID. Anal Biochem. 1964 May;8:126–129. doi: 10.1016/0003-2697(64)90177-0. [DOI] [PubMed] [Google Scholar]
  21. STEELE W. J., OKAMURA N., BUSCH H. EFFECTS OF THIOACETAMIDE ON THE COMPOSITION AND BIOSYNTHESIS OF NUCLEOLAR AND NUCLEAR RIBONUCLEIC ACID IN RAT LIVER. J Biol Chem. 1965 Apr;240:1742–1749. [PubMed] [Google Scholar]
  22. Soeiro R., Birnboim H. C., Darnell J. E. Rapidly labeled HeLa cell nuclear RNA. II. Base composition and cellular localization of a heterogeneous RNA fraction. J Mol Biol. 1966 Aug;19(2):362–372. doi: 10.1016/s0022-2836(66)80010-4. [DOI] [PubMed] [Google Scholar]
  23. Teng C. S., Hamilton T. H. The role of chromatin in estrogen action in the uterus, I. The control of template capacity and chemical composition and the binding of H3-estradiol-17 beta. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1410–1417. doi: 10.1073/pnas.60.4.1410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Willems M., Wagner E., Laing R., Penman S. Base composition of ribosomal RNA precursors in the HeLa cell nucleolus: further evidence of non-conservative processing. J Mol Biol. 1968 Mar 14;32(2):211–220. doi: 10.1016/0022-2836(68)90005-3. [DOI] [PubMed] [Google Scholar]

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