Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Apr;132(4):673–680. doi: 10.1042/bj1320673

Interrelationships in trace-element metabolism in metal toxicities in a cobalt-resistant strain of Neurospora crassa

G Venkateswerlu 1, K Sivarama Sastry 1
PMCID: PMC1177642  PMID: 4269100

Abstract

A strain of Neurospora crassa was isolated by training the mould to grow on media containing high concentrations of Co2+. This strain, the CoR strain, exhibited approximately tenfold the resistance of the parent strain to Co2+ and Ni2+ but not to Zn2+ or Cu2+. Co2+ toxicity in the CoR strain was reversed by Mg2+ but not by Fe3+. Also, Co2+ did not affect iron metabolism in this strain. It is suggested that the mechanism of resistance in the CoR strain involves an alteration in the pattern of iron metabolism such that the latter is no longer adversely affected by toxic concentrations of Co2+. The CoR strain is genetically stable and is most probably a result of a resistance mutation in N. crassa induced by Co2+.

Full text

PDF
673

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABELSON P. H., ALDOUS E. Ion antagonisms in microorganisms; interference of normal magnesium metabolism by nickel, cobalt, cadmium, zinc, and manganese. J Bacteriol. 1950 Oct;60(4):401–413. doi: 10.1128/jb.60.4.401-413.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADIGA P. R., SASTRY K. S., VENKATASUBRAMANYAM V., SARM P. S. Interrelationships in trace-element metabolism in Aspergillus niger. Biochem J. 1961 Dec;81:545–550. doi: 10.1042/bj0810545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ANTOINE A. LA R'ESISTANCE DE LA LEVURE AUX IONS CUIVRIQUES. II. SACCHAROMYCES CEREVISIAE, YEAST FOAM, INFLUENCE DES IONS CU2+ SUR L'APPARITION DE CELLULES R'ESISTANTES. Exp Cell Res. 1965 Feb;37:278–291. doi: 10.1016/0014-4827(65)90176-x. [DOI] [PubMed] [Google Scholar]
  4. ANTOINE A. LA RESISTANCE DE LA LEVURE AUX IONS CUIVRIQUES: SACCHAROMYCES ELLIPSOIDEUS. Exp Cell Res. 1964 Oct;36:73–85. doi: 10.1016/0014-4827(64)90161-2. [DOI] [PubMed] [Google Scholar]
  5. BRENES-POMALES A., LINDEGREN G., LINDEGREN C. C. Gene control of coppersensitivity in Saccharomyces. Nature. 1955 Oct 29;176(4487):841–842. doi: 10.1038/176841a0. [DOI] [PubMed] [Google Scholar]
  6. HEALY W. B., CHENG S. C., McELROY W. D. Metal toxicity and iron deficiency effects on enzymes in Neurospora. Arch Biochem Biophys. 1955 Jan;54(1):206–214. doi: 10.1016/0003-9861(55)90023-0. [DOI] [PubMed] [Google Scholar]
  7. LINDEGREN C. C., NAGAI S., NAGAI H. Induction of respiratory deficiency in yeast by manganese, copper, cobalt and nickel. Nature. 1958 Aug 16;182(4633):446–448. doi: 10.1038/182446a0. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. PADMANABAN G., SARMA P. S. A NEW IRON-BINDING COMPOUND FROM COBALT-TOXIC CULTURES OF NEUROSPORA CRASSA. Arch Biochem Biophys. 1964 Nov;108:362–363. doi: 10.1016/0003-9861(64)90403-5. [DOI] [PubMed] [Google Scholar]
  10. Padmanaban G., Sarma P. S. Cobalt toxicity and iron metabolism in Neurospora crassa. Biochem J. 1966 Jan;98(1):330–334. doi: 10.1042/bj0980330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SASTRY K. S., ADIGA P. R., VENKATASUBRAMANYAM V., SARMA P. S. Interrelationships in trace-element metabolism in metal toxicities in Neurospora crassa. Biochem J. 1962 Dec;85:486–491. doi: 10.1042/bj0850486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith D. H. R factors mediate resistance to mercury, nickel, and cobalt. Science. 1967 May 26;156(3778):1114–1116. doi: 10.1126/science.156.3778.1114. [DOI] [PubMed] [Google Scholar]
  13. Subramanian K. N., Padmanaban G., Sarma P. S. Folin-Ciocalteu reagent for the estimation of siderochromes. Anal Biochem. 1965 Jul;12(1):106–112. doi: 10.1016/0003-2697(65)90147-8. [DOI] [PubMed] [Google Scholar]
  14. Venkateswerlu G., Sastry K. S. The mechanism of uptake of cobalt ions by Neurospora crassa. Biochem J. 1970 Jul;118(3):497–503. doi: 10.1042/bj1180497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WEBBER B. B., DESERRES F. J. INDUCTION KINETICS AND GENETIC ANALYSIS OF X-RAY-INDUCED MUTATIONS IN THE AD-3 REGION OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1965 Feb;53:430–437. doi: 10.1073/pnas.53.2.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Webb M. Interrelationships between the utilization of magnesium and the uptake of other bivalent cations by bacteria. Biochim Biophys Acta. 1970 Nov 24;222(2):428–439. doi: 10.1016/0304-4165(70)90133-9. [DOI] [PubMed] [Google Scholar]
  17. Webb M. The mechanism of acquired resistance to Co2+ and Ni2+ in Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1970 Nov 24;222(2):440–446. doi: 10.1016/0304-4165(70)90134-0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES