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. 1973 Jun;133(2):329–334. doi: 10.1042/bj1330329

A collagenase in extracts of the invertebrate Bipalium kewense

Julius Phillips 1, Marc H Dresden 1
PMCID: PMC1177702  PMID: 4353237

Abstract

The localization, isolation and partial characterization of a collagenolytic enzyme from the land planarian Bipalium kewense is described. This enzyme can be obtained by direct extraction of the organism, and can be separated from non-collagenous proteolytic activity by (NH4)2SO4 precipitation and Sephadex-gel chromatography. Its mode of attack on collagen and sensitivity to a variety of inhibitors indicate that this enzyme differs from vertebrate collagenases and a previously described invertebrate collagenase.

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Selected References

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  1. Bauer E. A., Jeffrey J. J., Eisen A. Z. Preparation of three vertebrate collagenases in pure form. Biochem Biophys Res Commun. 1971 Aug 20;44(4):813–818. doi: 10.1016/0006-291x(71)90783-2. [DOI] [PubMed] [Google Scholar]
  2. Eisen A. Z., Jeffrey J. J. An extractable collagenase from crustacean hepatopancreas. Biochim Biophys Acta. 1969;191(3):517–526. doi: 10.1016/0005-2744(69)90345-3. [DOI] [PubMed] [Google Scholar]
  3. Fullmer H. M., Taylor R. E., Guthrie R. W. Human gingival collagenase: purification, molecular weight, and inhibitor studies. J Dent Res. 1972 Mar-Apr;51(2):349–355. doi: 10.1177/00220345720510022101. [DOI] [PubMed] [Google Scholar]
  4. GRASSMANN W., NORDWIG A. [Quantitative colorimetric test for collagenase]. Hoppe Seylers Z Physiol Chem. 1960 Dec 31;322:267–272. doi: 10.1515/bchm2.1960.322.1.267. [DOI] [PubMed] [Google Scholar]
  5. GROSS J. Studies on the formation of collagen. I. Properties and fractionation of neutral salt extracts of normal guinea pig connective tissue. J Exp Med. 1958 Feb 1;107(2):247–263. doi: 10.1084/jem.107.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harper E., Gross J. Separation of collagenase and peptidase activities of tadpole tissues in culture. Biochim Biophys Acta. 1970 Feb 11;198(2):286–292. doi: 10.1016/0005-2744(70)90061-6. [DOI] [PubMed] [Google Scholar]
  7. Harris E. D., Jr, DiBona D. R., Krane S. M. Collagenases in human synovial fluid. J Clin Invest. 1969 Nov;48(11):2104–2113. doi: 10.1172/JCI106177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JOSSE J., HARRINGTON W. F. ROLE OF PYRROLIDINE RESIDUES IN THE STRUCTURE AND STABILIZATION OF COLLAGEN. J Mol Biol. 1964 Aug;9:269–287. doi: 10.1016/s0022-2836(64)80207-2. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. MASER M. D., RICE R. V. Biophysical and biochemical properties of earthworm-cuticle collagen. Biochim Biophys Acta. 1962 Sep 24;63:255–265. doi: 10.1016/0006-3002(62)90679-0. [DOI] [PubMed] [Google Scholar]
  11. Miller R. L., Udenfriend S. Hydroxylation of proline residues in collagen nascent chains. Arch Biochem Biophys. 1970 Jul;139(1):104–113. doi: 10.1016/0003-9861(70)90051-2. [DOI] [PubMed] [Google Scholar]
  12. NAGAI Y., GROSS J., PIEZ K. A. DISC ELECTROPHORESIS OF COLLAGEN COMPONENTS. Ann N Y Acad Sci. 1964 Dec 28;121:494–500. doi: 10.1111/j.1749-6632.1964.tb14221.x. [DOI] [PubMed] [Google Scholar]
  13. Nagai Y., Lapiere C. M., Gross J. Tadpole collagenase. Preparation and purification. Biochemistry. 1966 Oct;5(10):3123–3130. doi: 10.1021/bi00874a007. [DOI] [PubMed] [Google Scholar]
  14. Peterkofsky B., Diegelmann R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971 Mar 16;10(6):988–994. doi: 10.1021/bi00782a009. [DOI] [PubMed] [Google Scholar]
  15. SINGLETON L. The chemical structure of earthworm cuticle. Biochim Biophys Acta. 1957 Apr;24(1):67–72. doi: 10.1016/0006-3002(57)90146-4. [DOI] [PubMed] [Google Scholar]
  16. Stark M., Kühn K. The properties of molecular fragments obtained on treating calfskin collagen with collagenase from Clostridium histolyticum. Eur J Biochem. 1968 Dec 5;6(4):534–541. doi: 10.1111/j.1432-1033.1968.tb00477.x. [DOI] [PubMed] [Google Scholar]
  17. WATSON M. R. The chemical composition of earthworm cuticle. Biochem J. 1958 Mar;68(3):416–420. doi: 10.1042/bj0680416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoshida E., Noda H. Isolation and characterization of collagenases I and II from Clostridium histolyticum. Biochim Biophys Acta. 1965 Sep 20;105(3):562–574. doi: 10.1016/s0926-6593(65)80239-9. [DOI] [PubMed] [Google Scholar]

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