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. 1973 Jul;133(3):507–513. doi: 10.1042/bj1330507

The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors

C R Lowe 1, M J Harvey 1, D B Craven 1, M A Kerfoot 1, M E Hollows 1, P D G Dean 1
PMCID: PMC1177728  PMID: 4147413

Abstract

1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and other interacting ligands can be made to selectively alter the chromatographic behaviour of the desired enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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