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. 1973 May;134(1):103–112. doi: 10.1042/bj1340103

A study of some enzymes of glycerolipid biosynthesis in rat liver after subtotal hepatectomy

E Heather Mangiapane 1,*, Katherine A Lloyd-Davies 1, David N Brindley 1
PMCID: PMC1177792  PMID: 4353080

Abstract

1. The accumulation of triglyceride in the liver remnant after subtotal hepatectomy (removal of 82% of the liver) exceeded that described for partial hepatectomy (removal of 70% of the liver). 2. Palmitoyl-CoA synthetase, glycerol phosphate acyltransferase and diglyceride acyltransferase activities were measured in the microsomal fraction, and phosphatidate phosphohydrolase activity was measured in the particle-free supernatant fraction, prepared from the liver remnant at various times after subtotal hepatectomy. 3. The only enzyme showing a significant change in specific activity was phosphatidate phosphohydrolase. The specific activity was approximately fivefold that of the control value at 6h after operation and threefold that of the control at 10, 16 and 24h after operation. A smaller increase in the specific activity of the enzyme in sham-operated animals occurred only at 6h after operation. 4. However, at this time the total phosphohydrolase activity of the remaining liver in the sham-operated rats was approximately threefold that in hepatectomized rats. 5. Injection of actinomycin D prevented the increase in activity of phosphatidate phosphohydrolase but did not prevent the accumulation of triglyceride.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aas M., Daae L. N. Fatty acid activation and acyl transfer in organs from rats in different nutritional states. Biochim Biophys Acta. 1971 Jul 13;239(2):208–216. doi: 10.1016/0005-2760(71)90166-4. [DOI] [PubMed] [Google Scholar]
  2. Alcindor L. G., Infante R., Soler-Argilaga C., Raisonnier A., Polonovski J., Caroli J. Induction of the hepatic synthesis of beta-lipoproteins by high concentrations of fatty acids. Effect of actinomycin D. Biochim Biophys Acta. 1970 Sep 8;210(3):483–486. doi: 10.1016/0005-2760(70)90045-7. [DOI] [PubMed] [Google Scholar]
  3. BARRON E. J., HANAHAN D. J. Observations on the silicic acid chromatography of the neutral lipides of rat liver, beef liver, and yeast. J Biol Chem. 1958 Mar;231(1):493–503. [PubMed] [Google Scholar]
  4. BEAUFAY H., DE DUVE C., HOLT S. J., UNDERHAY E. Intracellular localization of esterase in rat liver. J Biophys Biochem Cytol. 1956 Sep 25;2(5):635–637. doi: 10.1083/jcb.2.5.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Becker F. F. The normal hepatocyte in division: regeneration of the mammalian liver. Prog Liver Dis. 1970;3:60–76. [PubMed] [Google Scholar]
  6. Brindley D. N. The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa. Biochem J. 1973 Apr;132(4):707–715. doi: 10.1042/bj1320707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Camargo A. C., Cornicelli J., Cardoso S. S. Alteration in lipid content of the liver in the rat after partial hepatectomy. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1151–1154. doi: 10.3181/00379727-122-31349. [DOI] [PubMed] [Google Scholar]
  8. Delahunty T. J., Rubinstein D. Accumulation and release of triglycerides by rat liver following partial hepatectomy. J Lipid Res. 1970 Nov;11(6):536–543. [PubMed] [Google Scholar]
  9. Fallon H. J., Kemp E. L. Effects of diet on hepatic triglyceride synthesis. J Clin Invest. 1968 Apr;47(4):712–719. doi: 10.1172/JCI105766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Farstad M. In vivo and in vitro studies of the regulation of palmityl-CoA synthetase activity in rat liver. Acta Physiol Scand. 1968 Dec;74(4):568–576. doi: 10.1111/j.1748-1716.1968.tb04268.x. [DOI] [PubMed] [Google Scholar]
  11. Fex G., Olivecrona T. Secretion of triglyceride by the liver after partial hepatectomy. Biochim Biophys Acta. 1968 Oct 22;164(2):424–426. doi: 10.1016/0005-2760(68)90167-7. [DOI] [PubMed] [Google Scholar]
  12. Fex G., Olivecrona T. The metabolism of [3H]oleic acid in the partially hepatectomized rat. Biochim Biophys Acta. 1968 Mar 4;152(2):237–243. doi: 10.1016/0005-2760(68)90032-5. [DOI] [PubMed] [Google Scholar]
  13. Fex G. The metabolism of liver and plasma lipids after partial hepatectomy in the rat. Biochim Biophys Acta. 1970 May 5;202(3):415–425. doi: 10.1016/0005-2760(70)90112-8. [DOI] [PubMed] [Google Scholar]
  14. Girard A., Roheim P. S., Eder H. A. Lipoprotein synthesis and fatty acid mobilization in rats after partial hepatectomy. Biochim Biophys Acta. 1971 Oct 5;248(1):105–113. doi: 10.1016/0005-2760(71)90080-4. [DOI] [PubMed] [Google Scholar]
  15. Glaser G., Mager J. Biochemical studies on the mechanism of action of liver poisons. II. Induction of fatty livers. Biochim Biophys Acta. 1971 Feb 28;261(2):500–507. doi: 10.1016/0304-4165(72)90074-8. [DOI] [PubMed] [Google Scholar]
  16. Glende E. A., Jr, Morgan W. S. Alteration in liver lipid and lipid fatty acid composition after partial hepatectomy in the rat. Exp Mol Pathol. 1968 Apr;8(2):190–200. doi: 10.1016/0014-4800(68)90015-4. [DOI] [PubMed] [Google Scholar]
  17. Gravela E., Pani P., Ferrari A., Mazzarino C. Effects of the CCl 4 -cycloheximide interaction on protein synthesis and lipid metabolism in rat liver. Biochem Pharmacol. 1971 Dec;20(12):3423–3430. doi: 10.1016/0006-2952(71)90447-3. [DOI] [PubMed] [Google Scholar]
  18. Hübscher G., West G. R., Brindley D. N. Studies on the fractionation of mucosal homogenates from the small intestine. Biochem J. 1965 Dec;97(3):629–642. doi: 10.1042/bj0970629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Infante R., Alcindor G., Raisonnier A., Petit D., Polonovski J., Caroli J. Biosynthèse des lipoprotéines plasmatiques par le foie en régénération. Biochim Biophys Acta. 1969 Oct 28;187(3):335–344. [PubMed] [Google Scholar]
  20. JOHNSON R. M., ALBERT S. Glycerol- and acetate-C14 incorporation into lipids of tissues undergoing cell division. J Biol Chem. 1960 May;235:1299–1302. [PubMed] [Google Scholar]
  21. Lippel K. Regulation of rat liver acyl-CoA synthetase activity. Biochim Biophys Acta. 1971 Sep 1;239(3):384–392. doi: 10.1016/0005-2760(71)90031-2. [DOI] [PubMed] [Google Scholar]
  22. Mitchell M. P., Brindley D. N., Hübscher G. Properties of phosphatidate phosphohydrolase. Eur J Biochem. 1971 Jan;18(2):214–220. doi: 10.1111/j.1432-1033.1971.tb01233.x. [DOI] [PubMed] [Google Scholar]
  23. NIKKILAE E. A., OJALA K. ROLE OF HEPATIC L-ALPHA-GLYCEROPHOSPHATE AND TRIGLYCERIDE SYNTHESIS IN PRODUCTION OF FATTY LIVER BY ETHANOL. Proc Soc Exp Biol Med. 1963 Aug-Sep;113:814–817. doi: 10.3181/00379727-113-28499. [DOI] [PubMed] [Google Scholar]
  24. Olivecrona T., Fex G. Metabolism of plasma lipids in partially hepatectomized rats. Biochim Biophys Acta. 1970 Mar 10;202(2):259–268. doi: 10.1016/0005-2760(70)90187-6. [DOI] [PubMed] [Google Scholar]
  25. Park C. E., Marai E., Mookerjea S. Phospholipid metabolism in endoplasmic reticular membranes in rats fed high-carbohydrate diet. Biochim Biophys Acta. 1972 May 23;270(1):50–59. doi: 10.1016/0005-2760(72)90176-2. [DOI] [PubMed] [Google Scholar]
  26. STERN I., SHAPIRO B. A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood. J Clin Pathol. 1953 May;6(2):158–160. doi: 10.1136/jcp.6.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shephard E. H., Hübscher G. Phosphatidate biosynthesis in mitochondrial subfractions of rat liver. Biochem J. 1969 Jun;113(2):429–440. doi: 10.1042/bj1130429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Smith M. E., Hübscher G. The biosynthesis of glycerides by mitochondria from rat liver. The requirement for a soluble protein. Biochem J. 1966 Nov;101(2):308–316. doi: 10.1042/bj1010308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sánchez M., Nicholls D. G., Brindley D. N. [The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria]. Biochem J. 1973 Apr;132(4):697–706. doi: 10.1042/bj1320697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Vavrecka M., Mitchell M. P., Hübscher G. The effect of starvation on the incorporation of palmitate into glycerides and phospholipids of rat liver homogenates. Biochem J. 1969 Nov;115(2):139–145. doi: 10.1042/bj1150139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WEINBREN K., WOODWARD E. DELAYED INCORPORATION OF 32P FROM ORTHOPHOSPHATE INTO DEOXYRIBONUCLEIC ACID OF RAT LIVER AFTER SUBTOTAL HEPATECTOMY. Br J Exp Pathol. 1964 Aug;45:442–449. [PMC free article] [PubMed] [Google Scholar]

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