Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Jul;134(3):817–820.

Regulation of vitamin D metabolism: factors influencing the rate of formation of 1,25-dihydroxycholecalciferol by kidney homogenates (Short Communication)

Kay W Colston 1, Imogen M A Evans 1, Lee Galante 1, Iain MacIntyre 1, D W Moss 1
PMCID: PMC1177880  PMID: 4749279

Abstract

The formation of 1,25-dihydroxycholecalciferol from 25-hydroxycholecalciferol by chick kidney homogenates is inhibited by increasing concentrations of Ca2+. The apparent Km for the hydroxylation reaction is 1×10−7m, significantly lower than that reported for isolated mitochondria. Separated cytoplasmic and particulate fractions are inactive, but on recombination activity is restored, possibly because of the presence in the soluble fraction of a factor with a high affinity for 25-hydroxycholecalciferol.

Full text

PDF
817

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Fraser D. R., Kodicek E. Regulation of 25-hydroxycholecalciferol-1-hydroxylase activity in kidney by parathyroid hormone. Nat New Biol. 1973 Feb 7;241(110):163–166. doi: 10.1038/newbio241163a0. [DOI] [PubMed] [Google Scholar]
  3. Fraser D. R., Kodicek E. Unique biosynthesis by kidney of a biological active vitamin D metabolite. Nature. 1970 Nov 21;228(5273):764–766. doi: 10.1038/228764a0. [DOI] [PubMed] [Google Scholar]
  4. Galante L., Colston K., MacAuley S., MacIntyre I. Effect of parathyroid extract on vitamin-D metabolism. Lancet. 1972 May 6;1(7758):985–988. doi: 10.1016/s0140-6736(72)91156-7. [DOI] [PubMed] [Google Scholar]
  5. Gray R. W., Omdahl J. L., Ghazarian J. G., DeLuca H. F. 25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties. J Biol Chem. 1972 Dec 10;247(23):7528–7532. [PubMed] [Google Scholar]
  6. Haddad J. G., Chyu K. J. Competitive protein-binding radioassay for 25-hydroxycholecalciferol. J Clin Endocrinol Metab. 1971 Dec;33(6):992–995. doi: 10.1210/jcem-33-6-992. [DOI] [PubMed] [Google Scholar]
  7. Holick M. F., DeLuca H. F. A new chromatographic system for vitamin D3 and its metabolites: resoluation of a new vitamin D3 metabolite. J Lipid Res. 1971 Jul;12(4):460–465. [PubMed] [Google Scholar]
  8. Parsons J. A., Robinson C. J. Calcium shift into bone causing transient hypocalcaemia after injection of parathyroid hormone. Nature. 1971 Apr 30;230(5296):581–582. doi: 10.1038/230581a0. [DOI] [PubMed] [Google Scholar]
  9. Rasmussen H. Cell communication, calcium ion, and cyclic adenosine monophosphate. Science. 1970 Oct 23;170(3956):404–412. doi: 10.1126/science.170.3956.404. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES