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. 1973 Aug;134(4):885–889. doi: 10.1042/bj1340885

Effect of insulin on pyruvate metabolism in epididymal adipose tissue of the rat. Correlation of intracellular pyruvate contents and pyruvate dehydrogenase activity

Bruce G Berman 1, Mitchell L Halperin 1
PMCID: PMC1177896  PMID: 4762761

Abstract

A method is described to measure the intracellular content of pyruvate and lactate in epididymal adipose tissue of the rat. The intracellular pyruvate concentration was approx. 330μm. Intracellular pyruvate contents and the rates of pyruvate output were increased when NNN′N′-tetramethyl-p-phenylenediamine was added, and decreased in the presence of alanine. Insulin addition caused an increase in intracellular pyruvate contents only at the earlier time-period studied (1.5min as against 20min). Pyruvate dehydrogenase activity was increased in adipose tissue incubated in vitro with insulin. This increase occurred subsequent to the rise in the intracellular pyruvate content induced by insulin addition. The possible physiological implications are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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