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. 1973 Aug;134(4):985–993. doi: 10.1042/bj1340985

Hormonal regulation of glycogen metabolism in neonatal rat liver

Alan L Schwartz 1, Theodore W Rall 1
PMCID: PMC1177906  PMID: 4357717

Abstract

1. The development of active and inactive phosphorylase was determined in rat liver during the perinatal period. No inactive form could be found in tissues from animals less than 19 days gestation or older than the fifth postnatal day. 2. The regulation of phosphorylase in organ cultures of foetal rat liver was examined. None of the agents examined [glucagon, insulin or dibutyryl cyclic AMP (6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate)] changed the amount of phosphorylase activity. 3. Glycogen concentration in these explants were nevertheless decreased more than twofold by 4h of incubation with glucagon or dibutyryl cyclic AMP. Incubation with insulin for 4h increased the glycogen content twofold. 4. Glycogen synthetase activity was examined in these explants. I-form activity (without glucose 6-phosphate) was found to decrease by a factor of two after 4h of incubation with dibutyryl cyclic AMP, whereas I+D activity (with glucose 6-phosphate) remained nearly constant. Incubation for 4h with insulin increased I-form activity threefold, with only a slight increase in I+D activity. 5. When explants were incubated with insulin followed by addition of dibutyryl cyclic AMP, the effects of insulin on glycogen concentration and glycogen synthetase activity were reversed. 6. These results indicate that the regulation of glycogen synthesis may be the major factor in the hormonal control of glycogen metabolism in neonatal rat liver.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blázquez E., Montoya E., López Quijada C. Relationship between insulin concentrations in plasma and pancreas of oetal and weanling rats. J Endocrinol. 1970 Dec;48(4):553–561. doi: 10.1677/joe.0.0480553. [DOI] [PubMed] [Google Scholar]
  2. Cake M. H., Oliver I. T. The activation of phosphorylase in neonatal rat liver. Eur J Biochem. 1969 Dec;11(3):576–581. doi: 10.1111/j.1432-1033.1969.tb00809.x. [DOI] [PubMed] [Google Scholar]
  3. Craig J. W., Rall T. W., Larner J. The influence of insulin and epinephrine on adenosine 3',5'-phosphate and glycogen transferase in muscle. Biochim Biophys Acta. 1969 Apr 1;177(2):213–219. doi: 10.1016/0304-4165(69)90130-5. [DOI] [PubMed] [Google Scholar]
  4. DAWKINS M. J. GLYCOGEN SYNTHESIS AND BREAKDOWN IN FETAL AND NEWBORN RAT LIVER. Ann N Y Acad Sci. 1963 Dec 30;111:203–211. doi: 10.1111/j.1749-6632.1963.tb36960.x. [DOI] [PubMed] [Google Scholar]
  5. DAWKINS M. J. Glycogen synthesis and breakdown in rat liver at birth. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:265–272. doi: 10.1113/expphysiol.1963.sp001664. [DOI] [PubMed] [Google Scholar]
  6. Girard J., Bal D. Effets du glucagon-zinc sur la glycémie et la teneur en glycogène du foie foetal du rat en fin de gestation. C R Acad Sci Hebd Seances Acad Sci D. 1970 Aug 31;271(9):777–779. [PubMed] [Google Scholar]
  7. Greengard O., Dewey H. K. The premature deposition or lysis of glycogen in livers of fetal rats injected with hydrocortisone or glucagon. Dev Biol. 1970 Mar;21(3):452–461. doi: 10.1016/0012-1606(70)90135-1. [DOI] [PubMed] [Google Scholar]
  8. Hommes F. A., Beere A. The development of adenyl cyclase in rat liver, kidney, brain and skeletal muscle. Biochim Biophys Acta. 1971 May 18;237(2):296–300. doi: 10.1016/0304-4165(71)90321-7. [DOI] [PubMed] [Google Scholar]
  9. KREBS E. G., LOVE D. S., BRATVOLD G. E., TRAYSER K. A., MEYER W. L., FISCHER E. H. PURIFICATION AND PROPERTIES OF RABBIT SKELETAL MUSCLE PHOSPHORYLASE B KINASE. Biochemistry. 1964 Aug;3:1022–1033. doi: 10.1021/bi00896a003. [DOI] [PubMed] [Google Scholar]
  10. Kakiuchi S., Rall T. W. Studies on adenosine 3',5'-phosphate in rabbit cerebral cortex. Mol Pharmacol. 1968 Jul;4(4):379–388. [PubMed] [Google Scholar]
  11. Kornhauser D., Adam P. A., Schwartz R. Glucose production and utilization in the newborn puppy. Pediatr Res. 1970 Mar;4(2):120–128. doi: 10.1203/00006450-197003000-00002. [DOI] [PubMed] [Google Scholar]
  12. LEVINE R. A. EFFECT OF GLYCOGENOLYTIC AGENTS ON PHOSPHORYLASE ACTIVITY OF PERFUSED RAT LIVER. Am J Physiol. 1965 Feb;208:317–323. doi: 10.1152/ajplegacy.1965.208.2.317. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Novák E., Drummond G. I., Skála J., Hahn P. Developmental changes in cyclic AMP, protein kinase, phosphorylase kinase, and phosphorylase in liver, heart, and skeletal muscle of the rat. Arch Biochem Biophys. 1972 Jun;150(2):511–518. doi: 10.1016/0003-9861(72)90069-0. [DOI] [PubMed] [Google Scholar]
  15. RALL T. W., SUTHERLAND E. W., WOSILAIT W. D. The relationship of epinephrine and glucagon to liver phosphorylase. III. Reactivation of liver phosphorylase in slices and in extracts. J Biol Chem. 1956 Jan;218(1):483–495. [PubMed] [Google Scholar]
  16. SUTHERLAND E. W., CORI C. F. Effect of hyperglycemic-glycogenolytic factor and epinephrine on liver phosphorylase. J Biol Chem. 1951 Feb;188(2):531–543. [PubMed] [Google Scholar]
  17. Sato K., Morris H. P., Weinhouse S. Phosphorylase: a new isozyme in rat hepatic tumors and fetal liver. Science. 1972 Nov 24;178(4063):879–881. doi: 10.1126/science.178.4063.879. [DOI] [PubMed] [Google Scholar]
  18. Schwartz A. L. Influence of glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate and triamcinolone on the arginine synthetase system in perinatal rat liver. Biochem J. 1972 Jan;126(1):89–98. doi: 10.1042/bj1260089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thomas J. A., Schlender K. K., Larner J. A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucose. Anal Biochem. 1968 Oct 24;25(1):486–499. doi: 10.1016/0003-2697(68)90127-9. [DOI] [PubMed] [Google Scholar]
  20. Villar-Palasi C., Larner J., Shen L. C. Glycogen metabolism and the mechanism of action of cyclic AMP. Ann N Y Acad Sci. 1971 Dec 30;185:74–84. doi: 10.1111/j.1749-6632.1971.tb45238.x. [DOI] [PubMed] [Google Scholar]
  21. WOSILAIT W. D. Studies on the organic phosphate moiety of liver phosphorylase. J Biol Chem. 1958 Sep;233(3):597–600. [PubMed] [Google Scholar]

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