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. 1973 Aug;134(4):1045–1049. doi: 10.1042/bj1341045

Respiration-driven proton translocation by yeast mitochondria with differing efficiencies of oxidative phosphorylation

J A Downie 1, P B Garland 1
PMCID: PMC1177913  PMID: 4357710

Abstract

Measurements were made of the stoicheiometry of proton translocation coupled to respiration in mitochondria from Candida utilis where the number of functional energy-conservation sites between intramitochondrial NADH and oxygen was one in a mutant with a novel oxidase (Downie & Garland, 1972), two in sulphate-deficient cells (Haddock & Garland, 1971) or three in glycerol-limited cells (Light & Garland, 1971). The stoicheiometries of protons translocated per atom of oxygen utilized (i.e. →H+/2e− ratio; Mitchell, 1966) were close to 2.0, 4.0 and 6.0 respectively. Thus by using the same substrate (intramitochondrial NADH) and oxygen throughout, the →H+/2e− ratio is shown to be 2.0 per energy-conservation site when the number of such sites is varied from one to three.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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