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. 1973 Aug;134(4):1083–1091. doi: 10.1042/bj1341083

Biosynthesis of serum albumin in rat liver. Evidence for the existence of `proalbumin'

J D Judah 1, Margaret Gamble 1, J H Steadman 1
PMCID: PMC1177917  PMID: 4762753

Abstract

1. A protein(s) of rat liver (precipitated from soluble extracts of the microsomal fraction by anti-albumin) yields albumin after limited hydrolysis by trypsin. 2. Evidence that the product of limited tryptic hydrolysis is albumin, is based upon ion-exchange chromatography, electrofocusing and peptide `mapping'. 3. The albumin `precursor' is recognized by anti-albumin and is apparently not distinguished from albumin by anti-albumin. 4. A small peptide is liberated from the presumptive albumin precursor during limited tryptic hydrolysis. This peptide is labelled by arginine, but not by leucine, lysine or methionine. 5. These results support our previous suggestion based on kinetic evidence that the albumin-like protein(s), in the anti-albumin precipitate from rat liver, is an albumin precursor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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