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. 1971 Nov;125(1):189–201. doi: 10.1042/bj1250189

Determination of the sequences of protein-derived peptides and peptide mixtures by mass spectrometry

Howard R Morris 1, Dudley H Williams 1, Richard P Ambler 2
PMCID: PMC1178039  PMID: 5158904

Abstract

Micro-quantities of protein-derived peptides have been converted into N-acetylated permethyl derivatives, and their sequences determined by low-resolution mass spectrometry without prior knowledge of their amino acid compositions or lengths. A new strategy is suggested for the mass spectrometric sequencing of oligopeptides or proteins, involving gel filtration of protein hydrolysates and subsequent sequence analysis of peptide mixtures. Finally, results are given that demonstrate for the first time the use of mass spectrometry for the analysis of a protein-derived peptide mixture, again without prior knowledge of the protein or components within the mixture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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