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. 1972 Jan;126(1):27–34. doi: 10.1042/bj1260027

The purification and some properties of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from baker's yeast

B Middleton 1, P K Tubbs 1
PMCID: PMC1178348  PMID: 4561617

Abstract

1. A purification of 3-hydroxy-3-methylglutaryl-CoA synthase from baker's yeast is described. This yields a preparation of average specific activity 2.1 units (μmol/min)/mg in which contamination by acetoacetyl-CoA thiolase is less than 0.2%. 2. The molecular weights of 3-hydroxy-3-methylglutaryl-CoA synthase and acetoacetyl-CoA thiolase from baker's yeast were determined by gel filtration on Sephadex G-200. The values obtained were 130000 and 190000 respectively. 3. 3-Hydroxy-3-methylglutaryl-CoA synthase is susceptible to irreversible inhibition by a wide variety of alkylating and acylating agents. The time-course of inhibition of the enzyme by some of these, including the active-site-directed inhibitor bromoacetyl-CoA, was studied in the presence and absence of substrates, products and product analogues. Acetyl-CoA, even when present at concentrations as low as 5μm, gives almost complete protection. Other acyl-CoA derivatives give some protection, but only at concentrations 10–30-fold higher. 4. These results are discussed with reference to an ordered reaction pathway in which acetyl-CoA reacts to give a covalent acetyl-enzyme intermediate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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