Abstract
1. Three β-fructofuranosidases were separated by chromatography on a DEAE-cellulose column from the soluble protein extracted from dandelion (Taraxacum officinale Weber) roots. 2. One enzyme, which acted on sucrose, was characterized as an invertase, with a Km of 2.00×10−2m and pH optimum of 7.5. 3. The other two enzymes are hydrolases (A and B), which act on the inulin series of oligosaccharides [general formula glucose-fructose-(fructose)n]. They both have a pH optimum of 4.0 and Km of 1.54×10−2m but differ in their chromatographic behaviour on DEAE-cellulose. Neither of the hydrolases is inhibited by sucrose. 4. The physiological role of these three hydrolytic enzymes is discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BACON J. S. D., LOXLEY R. Seasonal changes in the carbohydrates of the Jerusalem Artichoke tuber. Biochem J. 1952 May;51(2):208–213. doi: 10.1042/bj0510208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman J., Jefford T. G. The metabolism of fructose polymers in plants. 4. Beta-fructofuranosidases of tubers of Helianthus tuberosus L. Biochem J. 1964 Oct;93(1):148–161. doi: 10.1042/bj0930148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PHELPS C. F. THE PHYSICAL PROPERTIES OF INULIN SOLUTIONS. Biochem J. 1965 Apr;95:41–47. doi: 10.1042/bj0950041. [DOI] [PMC free article] [PubMed] [Google Scholar]