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. 1972 Feb;126(3):675–681. doi: 10.1042/bj1260675

Comparison of the multiple deoxyribonucleic acid-dependent ribonucleic acid polymerase forms of whole rat liver and a minimal-deviation rat hepatoma cell line

C James Chesterton 1, Stuart M Humphrey 1, Peter H W Butterworth 2
PMCID: PMC1178425  PMID: 4342394

Abstract

To investigate the possibility that the pattern of multiple DNA-dependent RNA polymerases of an animal cell exerts a controlling influence on its nature, the activities of these enzymes were compared in differentiated rat liver and in a rapidly growing minimal-deviation rat hepatoma cell line by using established techniques of enzyme extraction, separation and determination. Relative to the DNA content of the tissues, RNA polymerase activities of forms AI, AII and B were approx. ninefold, twofold and twofold higher respectively in the cell line than in the liver. Tests indicated that these results could not be explained by differences in extraction efficiency or by the presence of unbound inhibitors or stimulators of polymerase activity in the final enzyme preparations. New forms of the enzyme were not detected in either tissue. The significance of these findings with respect to the possible role of multiple RNA polymerases in the control of cellular activities is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burgess R. R., Travers A. A., Dunn J. J., Bautz E. K. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. doi: 10.1038/221043a0. [DOI] [PubMed] [Google Scholar]
  3. Butterworth P. H., Cox R. F., Chesterton C. J. Transcription of mammalian chromatin by mammalian DNA-dependent RNA polymerases. Eur J Biochem. 1971 Nov 11;23(2):229–241. doi: 10.1111/j.1432-1033.1971.tb01613.x. [DOI] [PubMed] [Google Scholar]
  4. Chesterton C. J., Butterworth P. H. Selective extraction of form I DNA dependent RNA polymerase from rat liver nuclei and its separation into two species. Eur J Biochem. 1971 Mar 11;19(2):232–241. doi: 10.1111/j.1432-1033.1971.tb01309.x. [DOI] [PubMed] [Google Scholar]
  5. Chesterton C. J., Butterworth P. H.W. A new form of mammalian DNA-dependent RNA polymerase and its relationship to the known forms of the enzyme. FEBS Lett. 1971 Feb 9;12(6):301–308. doi: 10.1016/0014-5793(71)80001-7. [DOI] [PubMed] [Google Scholar]
  6. Chesterton C. J., Butterworth P. H.W. Purification of the rat liver form B DNA-dependent RNA polymerases. FEBS Lett. 1971 Jun 24;15(3):181–185. doi: 10.1016/0014-5793(71)80307-1. [DOI] [PubMed] [Google Scholar]
  7. KLEMPERER H. G. Manganous ion-dependent incorporation of adenosine-5'-phosphate into ribonucleic acid by an enzyme from rat liver. Biochim Biophys Acta. 1963 Jul 30;72:416–420. [PubMed] [Google Scholar]
  8. KLEMPERER H. G. The incorporation of uridine 5'-phosphate into ribonucleic acid by an enzyme preparation from rat liver. Biochim Biophys Acta. 1963 Jul 30;72:403–415. [PubMed] [Google Scholar]
  9. Kedinger C., Nuret P., Chambon P. Structural evidence for two alpha-amanitin sensitive RNA polymerases in calf thymus. FEBS Lett. 1971 Jun 24;15(3):169–174. doi: 10.1016/0014-5793(71)80305-8. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lindell T. J., Weinberg F., Morris P. W., Roeder R. G., Rutter W. J. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science. 1970 Oct 23;170(3956):447–449. doi: 10.1126/science.170.3956.447. [DOI] [PubMed] [Google Scholar]
  12. Mainwaring W. I., Mangan F. R., Peterken B. M. Studies on the solubilized ribonucleic acid polymerase from rat ventral prostate gland. Biochem J. 1971 Jul;123(4):619–628. doi: 10.1042/bj1230619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mandel J. L., Chambon P. Purification of RNA polymerase B activity from rat liver. FEBS Lett. 1971 Jun 24;15(3):175–180. doi: 10.1016/0014-5793(71)80306-x. [DOI] [PubMed] [Google Scholar]
  14. PITOT H. C., PERAINO C., MORSE P. A., Jr, POTTER V. R. HEPATOMAS IN TISSUE CULTURE COMPARED WITH ADAPTING LIVER IN VIVO. Natl Cancer Inst Monogr. 1964 Apr;13:229–245. [PubMed] [Google Scholar]
  15. Reel J. R., Kenney F. T. "Superinduction" of tyrosine transaminase in hepatoma cell cultures: differential inhibition of synthesis and turnover by actionomycin D. Proc Natl Acad Sci U S A. 1968 Sep;61(1):200–206. doi: 10.1073/pnas.61.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richardson J. P. Some physical properties of RNA polymerase. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1616–1623. doi: 10.1073/pnas.55.6.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roeder R. G., Rutter W. J. Specific nucleolar and nucleoplasmic RNA polymerases. Proc Natl Acad Sci U S A. 1970 Mar;65(3):675–682. doi: 10.1073/pnas.65.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stein H., Hausen P. A factor from calf thymus stimulating DNA-dependent RNA polymerase isolated from this tissue. Eur J Biochem. 1970 Jun;14(2):270–277. doi: 10.1111/j.1432-1033.1970.tb00286.x. [DOI] [PubMed] [Google Scholar]
  19. Tocchini-Valentini G. P., Crippa M. Ribosomal RNA synthesis and RNA polymerase. Nature. 1970 Dec 5;228(5275):993–995. doi: 10.1038/228993a0. [DOI] [PubMed] [Google Scholar]

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