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. 1972 Feb;126(4):781–790. doi: 10.1042/bj1260781

The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid

N W Penn 1,*, R Suwalski 1, C O'Riley 1, K Bojanowski 1, R Yura 1,†
PMCID: PMC1178489  PMID: 4538516

Abstract

A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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