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. 1972 Mar;127(1):1–10. doi: 10.1042/bj1270001

The deoxyribonucleic acid modification enzyme of bacteriophage P1. Purification and properties

Jeremy P Brockes 1, Paul R Brown 2, K Murray 2
PMCID: PMC1178554  PMID: 5073742

Abstract

The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20–24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0–6.25: the apparent Km for S-adenosyl-l-methionine is 5×10−6m.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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