Abstract
1. The biosynthesis of the wall teichoic acid, poly(glycerol phosphate glucose), has been studied with a particulate membrane preparation from Bacillus licheniformis A.T.C.C. 9945. The precursor CDP-glycerol supplies glycerol phosphate residues, whereas UDP-glucose supplies only glucose to the repeating structure of the polymer. 2. Synthesis proceeds through polyprenol phosphate derivatives, and chemical studies and pulse-labelling techniques show that the first intermediate is the phosphodiester, glucose polyprenol monophosphate. CDP-glycerol donates a glycerol phosphate residue to this to give a second intermediate, (glycerol phosphate glucose phosphate) polyprenol. 3. The glucose residue in the lipid intermediates has the β configuration, and chain extension in the synthesis of polymer occurs by transglycosylation with inversion of anomeric configuration at two stages.
Full text
PDF










Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson R. G., Hussey H., Baddiley J. The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane. Biochem J. 1972 Mar;127(1):11–25. doi: 10.1042/bj1270011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Archibald A. R., Baddiley J., Blumsom N. L. The teichoic acids. Adv Enzymol Relat Areas Mol Biol. 1968;30:223–253. doi: 10.1002/9780470122754.ch5. [DOI] [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Baddiley J., Blumsom N. L., Douglas L. J. The biosynthesis of the wall teichoic acid in Staphylococcus lactis I3. Biochem J. 1968 Dec;110(3):565–571. doi: 10.1042/bj1100565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks D., Baddiley J. A lipid intermediate in the synthesis of a poly-(N-acetylglucosamine 1-phosphate) from the wall of Staphylococcus lactis N.C.T.C. 2102. Biochem J. 1969 Nov;115(2):307–314. doi: 10.1042/bj1150307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burger M. M., Glaser L. The synthesis of teichoic acids. V. Polyglucosylglycerol phosphate and polygalactosylglycerol phosphate. J Biol Chem. 1966 Jan 25;241(2):494–506. [PubMed] [Google Scholar]
- Douglas L. J., Baddiley J. A lipid intermediate in the biosynthesis of a teichoic acid. FEBS Lett. 1968 Aug;1(2):114–116. doi: 10.1016/0014-5793(68)80034-1. [DOI] [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- Hughes A. H., Stow M., Hancock I. C., Baddiley J. Function of teichoic acids and effect of novobiocin on control of Mg2+ at the bacterial membrane. Nat New Biol. 1971 Jan 13;229(2):53–55. doi: 10.1038/newbio229053a0. [DOI] [PubMed] [Google Scholar]
- Nikaido K., Nikaido H. Glucosylation of lipopolysaccharide in Salmonella: biosynthesis nof O antigen factor n12 2 . II. Structure of the lipid intermediate. J Biol Chem. 1971 Jun 25;246(12):3912–3919. [PubMed] [Google Scholar]
- PALADINI A. C., LELOIR L. F. Studies on uridine-diphosphate-glucose. Biochem J. 1952 Jun;51(3):426–430. doi: 10.1042/bj0510426. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scher M., Lennarz W. J., Sweeley C. C. The biosynthesis of mannosyl-1-phosphoryl-polyisoprenol in Micrococcus lysodeikticus and its role in mannan synthesis. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1313–1320. doi: 10.1073/pnas.59.4.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw N. Bacterial glycolipids. Bacteriol Rev. 1970 Dec;34(4):365–377. doi: 10.1128/br.34.4.365-377.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F. Biosynthesis of glucosyl diglycerides by Mycoplasma laidlawii strain B. J Bacteriol. 1969 Aug;99(2):480–486. doi: 10.1128/jb.99.2.480-486.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutherland I. W., Norval M. The synthesis of exopolysaccharide by Klebsiella aerogenes membrane preparations and the involvement of lipid intermediates. Biochem J. 1970 Dec;120(3):567–576. doi: 10.1042/bj1200567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
- Troy F. A., Frerman F. E., Heath E. C. The biosynthesis of capsular polysaccharide in Aerobacter aerogenes. J Biol Chem. 1971 Jan 10;246(1):118–133. [PubMed] [Google Scholar]
- Watkinson R. J., Hussey H., Baddiley J. Shared lipid phosphate carrier in the biosynthesis of teichoic acid and peptidoglycan. Nat New Biol. 1971 Jan 13;229(2):57–59. doi: 10.1038/newbio229057a0. [DOI] [PubMed] [Google Scholar]
- Wright A., Dankert M., Fennessey P., Robbins P. W. Characterization of a polyisoprenoid compound functional in O-antigen biosynthesis. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1798–1803. doi: 10.1073/pnas.57.6.1798. [DOI] [PMC free article] [PubMed] [Google Scholar]