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. 1970 Mar;117(1):9–15. doi: 10.1042/bj1170009

Effect of temperature acclimatization on the fatty acid composition of goldfish intestinal lipids

P Kemp 1, M W Smith 1
PMCID: PMC1178825  PMID: 4316092

Abstract

1. The fatty acid composition of whole goldfish, whole-intestinal mucosa, intestinal mucosal membranes and individual phospholipids extracted from mucosal membranes were measured, fish adapted to different temperatures being used. 2. Alterations of the adaptation temperature did not noticeably affect the fatty acid composition of the whole-fish lipids, but there were marked changes in the fatty acids of lipids extracted from homogenates of goldfish intestinal mucosa. These changes were more pronounced in a membrane fraction prepared from these homogenates. Raising the adaptation temperature by 20°C halved the percentage of C20:1, C20:4 and C22:6 fatty acids and nearly doubled the percentage of C18:0 and C20:3 fatty acids recovered. 3. Choline phosphoglycerides constituted about one-half and ethanolamine phosphoglycerides about one-quarter of the total membrane phospholipids. 4. The fatty acids of choline and ethanolamine phosphoglycerides were more susceptible to temperature-dependent changes than were the phosphoglycerides of inositol or serine. 5. The increase in C18:0 fatty acid that occurred in membranes of warm-adapted fish was greatest for ethanolamine phosphoglycerides, but increases also occurred in other phospholipid fractions and in membrane neutral lipids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CARROLL K. K. Quantitative estimation of peak areas in gas-liquid chromatography. Nature. 1961 Jul 22;191:377–378. doi: 10.1038/191377a0. [DOI] [PubMed] [Google Scholar]
  2. Chapman D. Physical studies of lipid-lipid and lipid-protein interactions. Lipids. 1969 Jul;4(4):251–260. doi: 10.1007/BF02533182. [DOI] [PubMed] [Google Scholar]
  3. Eibl H., Hill E. E., Lands W. E. The subcellular distribution of acyltransferases which catalyze the synthesis of phosphoglycerides. Eur J Biochem. 1969 Jun;9(2):250–258. doi: 10.1111/j.1432-1033.1969.tb00602.x. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Hyodo Y. Development of intestinal damage after x-irradiation and H3-thymidine incorporation into intestinal epithelial cells of irradiated goldfish, Carassius auratus, at different temperatures. Radiat Res. 1965 Nov;26(3):383–394. [PubMed] [Google Scholar]
  6. JOHNSTON P. V., ROOTS B. I. BRAIN LIPID FATTY ACIDS AND TEMPERATURE ACCLIMATION. Comp Biochem Physiol. 1964 Mar;11:303–309. doi: 10.1016/0010-406x(64)90111-2. [DOI] [PubMed] [Google Scholar]
  7. Knipprath W. G., Mead J. F. The effect of the environmental temperature on the fatty acid composition and on thein vivo incorporation of 1-(14)C-acetate in goldfish (Carassius auratus L.). Lipids. 1968 Mar;3(2):121–128. doi: 10.1007/BF02531728. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Roots B. I. Phospholipids of goldfish (Carassius auratus L.) brain: the influence of environmental temperature. Comp Biochem Physiol. 1968 May;25(2):457–466. doi: 10.1016/0010-406x(68)90354-x. [DOI] [PubMed] [Google Scholar]
  10. Smith M. W., Colombo V. E., Munn E. A. Influence of temperature acclimatization on the ionic activation of goldfish intestinal adenosine triphosphatase. Biochem J. 1968 May;107(5):691–698. doi: 10.1042/bj1070691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith M. W. Influence of temperature acclimatization on the temperature-dependence and ouabain-sensitizing of goldfish intestinal adenosine triphosphatase. Biochem J. 1967 Oct;105(1):65–71. doi: 10.1042/bj1050065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith M. W., Kemp P. Phospholipase C-induced changes in intestinal adenosine triphosphatase prepared from goldfish acclimatized to different temperatures. Biochem J. 1969 Sep;114(3):659–661. doi: 10.1042/bj1140659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vaskovsky V. E., Kostetsky E. Y. Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res. 1968 May;9(3):396–396. [PubMed] [Google Scholar]

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