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. 1970 Apr;117(2):397–403. doi: 10.1042/bj1170397

Studies on the competence-inducing factor of Bacillus subtilis

A Akrigg 1, S R Ayad 1
PMCID: PMC1178873  PMID: 4986873

Abstract

1. Aqueous extracts of competent cells of Bacillus subtilis 168I− were shown to contain a competence-inducing factor. The aqueous extracts were fractionated on DEAE-cellulose columns. 2. Those fractions from DEAE-cellulose columns containing the competence-inducing factor were shown to exhibit a powerful lytic effect on isolated cell walls of B. subtilis 168I−. Little or no lytic activity was exhibited by the other fractions. 3. The kinetics of the lytic enzyme were investigated and found to be first-order. Treatment of cell wall lysates with 1-fluoro-2,4-dinitrobenzene suggested that the enzyme may be identical with N-acetylmuramyl-l-alanine amidase. The amino acid composition of the partially purified enzyme was determined. 4. It is suggested that competence-induction may be dependent on the limited action of the autolytic amidase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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